In many biomedical and biotechnological applications, proteins are immobilized at aqueous-solid interfaces. Generally, an adsorption-induced denaturation of the proteins has to be avoided, because it prevents the use of their biological activity or it might cause adverse effects in living organisms. Furthermore, control over the degree of protein adsorption including protein desorption is extremely useful. Over the recent years, polyelectrolyte brushes have been examined in terms of protein adsorption, and the majority of the studies document their biocompatible properties. Moreover, they show a variable protein affinity responding to the ionic strength of the protein solution. In this short review, selected studies are highlighted that illustrate the most significant properties of polyelectrolyte brushes as adsorbent interfacial structure for proteins. Graphical abstract Proteins embedded into dense polyelectrolyte brushes.

中文翻译：

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蛋白质在聚电解质刷上的吸附机制、二级结构和局部分布

在许多生物医学和生物技术应用中，蛋白质被固定在水-固界面上。通常，必须避免蛋白质的吸附诱导变性，因为这会阻止其生物活性的使用，或者可能对生物体造成不利影响。此外，对包括蛋白质解吸在内的蛋​​白质吸附程度的控制非常有用。近年来，聚电解质刷已在蛋白质吸附方面进行了检查，大多数研究记录了它们的生物相容性。此外，它们对蛋白质溶液的离子强度显示出可变的蛋白质亲和力。在这篇简短的评论中，选定的研究突出显示了聚电解质刷作为蛋白质吸附界面结构的最重要特性。嵌入致密聚电解质画笔中的图形抽象蛋白质。