Abstract Aspergillus ochraceus (A. ochraceus) infection can cause grape decay and nutritional quality loss during postharvest storage. Previous studies showed that Melaleuca alternifolia oil (MAO) had strong anti-fungal effects, however, research on the effects of MAO on grape metabolisms is lacking. In this study, effects of MAO on mycelial growth and OTA production of A. ochraceus and metabolites of grapes were studied. Results showed that MAO significantly inhibited the mycelial growth and spore germination of A. ochraceus with the increase of concentration. MAO reduced the accumulation of OTA by downregulating the gene of OTA biosynthesis pathway. MAO can alleviate the down-regulation of some metabolites such as sugars and polyphenols in tricarboxylic acid cycle, glycolysis pathway, pentose phosphate pathway and phenols pathway in grape berries infected by A. ochraceus. The results of qRT-PCR showed that the decrease of metabolites caused by A. ochraceus was due to the down-regulation of genes related to those metabolites biosynthesis, but MAO could delay this situation. Therefore, MAO may be used as an alternative preservative for controlling the destruction of nutritional quality of grapes caused by A. ochraceus after harvest.

中文翻译：

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互叶白千层油可通过调控代谢途径中的关键基因和代谢物，延缓赭曲霉对葡萄的营养损伤

摘要 Aspergillus ochraceus (A. ochraceus) 感染可导致葡萄在采后贮藏期间腐烂和营养品质下降。以往的研究表明互叶白千层油（MAO）具有很强的抗真菌作用，但目前尚缺乏关于 MAO 对葡萄代谢影响的研究。在这项研究中，研究了 MAO 对 A. ochraceus 菌丝生长和 OTA 生产以及葡萄代谢物的影响。结果表明，随着浓度的增加，MAO显着抑制赭曲霉的菌丝生长和孢子萌发。MAO通过下调OTA生物合成途径的基因来减少OTA的积累。MAO可以减轻三羧酸循环、糖酵解途径中糖类和多酚等代谢物的下调，A. ochraceus 感染葡萄浆果中的磷酸戊糖途径和酚类途径。qRT-PCR结果表明，A. ochraceus引起的代谢物减少是由于与这些代谢物生物合成相关的基因下调，但MAO可以延缓这种情况。因此，MAO 可作为替代防腐剂用于控制葡萄采收后由赭曲霉对营养品质造成的破坏。