Photoacoustic microscopy (PAM) provides a new method for the imaging of small‐animals with high‐contrast and deep‐penetration. However, the established PAM systems have suffered from a limited field‐of‐view or imaging speed, which are difficult to both monitor wide‐field activity of organ and record real‐time change of local tissue. Here, we reported a dual‐raster‐scanned photoacoustic microscope (DRS‐PAM) that integrates a two‐dimensional motorized translation stage for large field‐of‐view imaging and a two‐axis fast galvanometer scanner for real‐time imaging. The DRS‐PAM provides a flexible transition from wide‐field monitoring the vasculature of organs to real‐time imaging of local dynamics. To test the performance of DRS‐PAM, clear characterization of angiogenesis and functional detail was illustrated, hemodynamic activities of vasculature in cerebral cortex of a mouse were investigated. Furthermore, response of tumor to treatment were successfully monitored during treatment. The experimental results demonstrate the DRS‐PAM holds the great potential for biomedical research of basic biology.

中文翻译：

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利用双光栅扫描光声显微镜对小动物的微血管网络进行宽域监控和实时本地记录。

光声显微镜（PAM）为高对比度和深穿透率的小动物成像提供了一种新方法。但是，已建立的PAM系统的视野或成像速度受到限制，难以同时监视器官的广域活动和记录局部组织的实时变化。在这里，我们报道了一种双光栅扫描光声显微镜（DRS-PAM），该显微镜集成了用于大视野成像的二维电动平移台和用于实时成像的两轴快速振镜扫描仪。DRS‐PAM提供了从大范围监视器官血管系统到实时局部动态成像的灵活过渡。为了测试DRS‐PAM的性能，对血管生成和功能细节进行了清晰的描述，研究了小鼠大脑皮层脉管系统的血流动力学活性。此外，在治疗期间成功监测了肿瘤对治疗的反应。实验结果表明，DRS‐PAM在基础生物学的生物医学研究中具有巨大的潜力。