Abstract Enzymatic processes are emerging as important green biotechnological processes in textile industry. The application of recombinant pectin methylesterase (CtPME) and pectate lyase (CtPL1B) from Clostridium thermocellum for enzymatic degumming of jute or bioscouring of cotton was evaluated. The effectiveness of processes by combination of two enzymes were evaluated that effective degumming of jute and bioscouring of cotton as compared with individual enzyme. The optimum concentrations of two enzymes mixture for both processes, degumming of jute and bio scouring of cotton were 5 mg/mL (2.1 U/mL) of CtPME and 5 mg/mL (3.0 U/mL) of CtPL1B under optimized conditions of 60 min, 100 rpm and 50 °C. FESEM images showed more effective removal of pectin from jute fiber and cotton fabric by enzyme mixture, nevertheless similar to NaOH treatment. Wettability analysis showed mixture of enzymes and NaOH treated cotton fabric absorbed a water drop in 10 s and 8 s, respectively. UTM analysis showed higher tensile strength and Young’s modulus for jute fiber and cotton fabric treated with enzyme mixture than untreated and were similar to those of NaOH treated. These results showed that the CtPME and CtPL1B mixture can be used for replacing the chemical process by green bioprocess in textile industry.

中文翻译：

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热纤梭菌重组果胶甲酯酶和果胶酸裂解酶对黄麻纤维脱胶和棉织物生物煮练的绿色生物工艺

摘要 酶法作为纺织工业中重要的绿色生物技术过程正在兴起。评估了来自热纤梭菌的重组果胶甲酯酶 (CtPME) 和果胶酸裂解酶 (CtPL1B) 在黄麻酶促脱胶或棉花生物煮练中的应用。与单独的酶相比，评估了两种酶组合工艺的有效性，即黄麻的有效脱胶和棉花的生物煮练。两种酶混合物在黄麻脱胶和棉花生物煮练过程中的最佳浓度为 5 mg/mL (2.1 U/mL) CtPME 和 5 mg/mL (3.0 U/mL) CtPL1B，优化条件为 60分钟，100 rpm 和 50 °C。FESEM 图像显示酶混合物更有效地去除黄麻纤维和棉织物中的果胶，但与 NaOH 处理类似。润湿性分析表明，酶和 NaOH 处理的棉织物的混合物分别在 10 秒和 8 秒内吸收了一滴水。UTM 分析表明，酶混合物处理的黄麻纤维和棉织物的拉伸强度和杨氏模量高于未处理的，并且与 NaOH 处理的相似。这些结果表明，CtPME 和 CtPL1B 混合物可用于在纺织工业中用绿色生物过程代替化学过程。