Abstract

Unsuitable pH is a major limiting factor for all organisms, and a low pH can lead to organism death. Late embryogenesis abundant (LEA) peptides confer tolerance to abiotic stresses including salinity, drought, high and low temperature, and ultraviolet radiation same as the LEA proteins from which they originate. In this study, LEA peptides derived from group 3 LEA proteins of Polypedilum vanderplanki were used to enhance low pH tolerance. Recombinant Escherichia coli BL21 (DE3) cells expressing the five designed LEA peptides were grown at pH 4, 3, and 2. The transformants showed higher growth capacity at low pH as compared to control cells. These results indicate that LEA peptide could prevent E. coli cell death under low pH conditions.

中文翻译：

---

从后期胚胎发生时设计的一种短肽，富含蛋白质，可增强大肠杆菌的耐酸性。

摘要

pH值不合适是所有生物的主要限制因素，低pH值可能导致生物死亡。晚期胚胎发生丰富（LEA）肽赋予非生物胁迫耐受性，包括盐度，干旱，高温和低温以及与它们起源的LEA蛋白质相同的紫外线辐射。在这项研究中，使用了多枝ped3个LEA蛋白衍生的LEA肽来增强低pH耐受性。表达五个设计的LEA肽的重组大肠杆菌BL21（DE3）细胞在pH 4、3和2下生长。与对照细胞相比，转化子在低pH下显示出更高的生长能力。这些结果表明，LEA肽可以防止在低pH条件下大肠杆菌的死亡。