Single-cell RNA sequencing has emerged as a powerful tool for characterizing cells, but not all phenotypes of interest can be observed through changes in gene expression. Linking sequencing with optical analysis has provided insight into the molecular basis of cellular function, but current approaches have limited throughput. Here, we present a high-throughput platform for linked optical and gene expression profiling of single cells. We demonstrate accurate fluorescence and gene expression measurements on thousands of cells in a single experiment. We use the platform to characterize DNA and RNA changes through the cell cycle and correlate antibody fluorescence with gene expression. The platform’s ability to isolate rare cell subsets and perform multiple measurements, including fluorescence and sequencing-based analysis, holds potential for scalable multi-modal single-cell analysis.

中文翻译：

---

高通量单细胞的关联光学和基因表达谱分析

单细胞 RNA 测序已成为表征细胞的强大工具，但并非所有感兴趣的表型都可以通过基因表达的变化来观察。将测序与光学分析联系起来可以深入了解细胞功能的分子基础，但目前的方法吞吐量有限。在这里，我们提出了一个用于单细胞链接光学和基因表达谱分析的高通量平台。我们在一次实验中展示了对数千个细胞的准确荧光和基因表达测量。我们使用该平台来表征细胞周期中 DNA 和 RNA 的变化，并将抗体荧光与基因表达相关联。该平台能够分离稀有细胞亚群并执行多种测量，包括基于荧光和测序的分析，具有可扩展的多模式单细胞分析的潜力。