BACKGROUND The polyketide antibiotic 2,4-diacetylphloroglucinol (2,4-DAPG), produced by Pseudomonas fluorescens 2P24, is positively regulated by the GacS-GacA two-component system. RESULTS Here we reported on the characterization of DsbA1 (disulfide oxidoreductase) as novel regulator of biocontrol activity in P. fluorescens. Our data showed that mutation of dsbA1 caused the accumulation of 2,4-DAPG in a GacA-independent manner. Further analysis indicated that DsbA1 interacts with membrane-bound glucose dehydrogenase Gcd, which positively regulates the production of 2,4-DAPG. Mutation of cysteine (C)-235, C275, and C578 of Gcd, significantly reduced the interaction with DsbA1, enhanced the activity of Gcd and increased 2,4-DAPG production. CONCLUSIONS Our results suggest that DsbA1 regulates the 2,4-DAPG concentration via fine-tuning the function of Gcd in P. fluorescens 2P24.

中文翻译：

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氧化还原酶DsbA1通过干扰Gcd在荧光假单胞菌2P24中的功能，对2,4-二乙酰基间苯三酚的生物合成产生负面影响。

背景技术荧光假单胞菌（Pseudomonasfluorescens）2P24产生的聚酮化合物抗生素2，4-二乙酰基间苯三酚（2，4-DAPG）受到GacS-GacA两组分系统的正调控。结果在这里我们报道了DsbA1（二硫键氧化还原酶）作为荧光假单胞菌中新的生物防治活性调节剂的特征。我们的数据表明，dsbA1突变以不依赖GacA的方式引起2,4-DAPG的积累。进一步的分析表明，DsbA1与膜结合的葡萄糖脱氢酶Gcd相互作用，后者积极调节2，4-DAPG的产生。Gcd的半胱氨酸（C）-235，C275​​和C578的突变显着降低了与DsbA1的相互作用，增强了Gcd的活性并增加了2,4-DAPG的产生。结论我们的结果表明DsbA1调节2，