Background Aneuploidy, a condition caused by an imbalance between the relative dosages of chromosomes, generally produces a novel phenotype specific to the molecular karyotype. Few techniques are currently available for detecting the molecular karyotypes of aneuploids in plants. Results Based on this imbalance in chromosome dosage, a new approach (referred to as 'SSR-qPCR') combining simple sequence repeat (SSR) markers and quantitative real-time PCR (qPCR) has been developed and utilized to detect some common aneuploids irrespective of heterozygosity. We screened 17 specific SSR markers covering all loquat linkage groups and redesigned 6 pairs of primers for SSR markers that can detect loquat chromosome aneuploidies. The SSR-qPCR detection results obtained for hybrid progeny and open-pollination progeny of triploid loquat showed diagnostic accuracies of 88.9% and 62.5%, respectively, compared with the chromosome preparation results. Conclusion SSR-qPCR can detect loquat aneuploids and be used to construct the entire molecular karyotypes of aneuploid individuals. Therefore, this method offers a novel alternative for the detection of chromosome aneuploidies.

中文翻译：

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枇杷（Eriobotrya japonica）非整倍体的分子核型可以通过使用SSR标记结合定量PCR来检测，而不管杂合性如何。

背景 非整倍性是由染色体的相对剂量之间的不平衡引起的一种状况，通常会产生特定于分子核型的新表型。目前很少有技术可用于检测植物中非整倍体的分子核型。结果基于染色体剂量的这种不平衡，一种新的方法（称为“SSR-qPCR”）结合简单序列重复（SSR）标记和定量实时PCR（qPCR）已被开发并用于检测一些常见的非整倍体。的杂合性。我们筛选了17个覆盖所有枇杷连锁群的特异性SSR标记，并重新设计了6对可检测枇杷染色体非整倍体的SSR标记引物。三倍体枇杷杂交后代和开放授粉后代的SSR-qPCR检测结果与染色体制备结果相比，诊断准确率分别为88.9%和62.5%。结论 SSR-qPCR可检测枇杷非整倍体，可用于构建非整倍体个体的完整分子核型。因此，该方法为检测染色体非整倍体提供了一种新的替代方法。