Compounds containing vicinal diol (glycol) groups, including saccharides, could be modified with sixvalent osmium complexes with nitrogenous ligands, particularly with N,N,N',N'-tetramethylethylenediamine (Os(VI)tem). The modification products are electrochemically active. Here we show that aminosaccharides can also be modified by Os(VI)tem. We studied chitosan oligosaccharides in their acetylated and deacetylated form in 0.2 M Na-phosphate, pH 6.9. Deacetylated chitosan oligosaccharides with free amino groups modified by Os(VI)tem yielded two peaks (peak I' at -0.15 V and peak II' at about -0.38 V) despite the fact that these oligomers contain only one glycol group on the non-reducing end of the molecule. The electrochemical behavior of Os(VI)tem modified deacetylated chitosan oligomers differs from Os(VI)tem modified simple saccharides, containing only glycol groups, predominantly in peak I'. Our results suggest that free amino groups are involved in Os(VI)tem modification of chitosan oligomers.

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tetra（VI）四甲基乙二胺修饰的壳寡糖的循环和方波伏安法

含有邻二醇（乙二醇）基团的化合物（包括糖类）可以用具有含氮配体的六价complex配合物，特别是N，N，N'，N'-四甲基乙二胺（Os（VI）tem）进行修饰。改性产物具有电化学活性。在这里，我们显示氨基糖也可以被Os（VI）tem修饰。我们研究了在0.2 M磷酸钠，pH 6.9下的乙酰化和脱乙酰化形式的壳聚糖低聚糖。Os（VI）tem修饰的具有游离氨基的脱乙酰壳聚糖寡糖产生两个峰（峰I'在-0.15 V，峰II'在约-0.38 V），尽管这些低聚物在非-上仅包含一个乙二醇基团还原分子的末端。Os（VI）tem修饰的脱乙酰壳聚糖低聚物的电化学行为不同于Os（VI）tem修饰的仅包含乙二醇基团的简单糖，主要在峰I'中。我们的结果表明，游离氨基与壳聚糖低聚物的Os（VI）tem修饰有关。