Considerably stable enzymatic fuel‐cells (single cell and 5‐cells stack) were prepared by using chitosan based membranes along with glucose oxidase attached bioanode. Continuous operation of fuel‐cells were monitored under short circuit conditions reaching half‐life over a week. Detailed analysis for the effects of pH, temperature, buffer types and concentration on different type of in‐house produced chitosan membranes were performed by electrochemical impedance spectroscopy (EIS). EIS was utilized to observe, total electrolyte resistance, charge transfer properties, mass transfer and double layer effects on integrated fuel cells (single cell and 5‐cells stack). Performance of the fuel cells was also analyzed by the polarization experiments. Current density of the fuel cell increased at higher operation temperatures not only due to better enzyme kinetics, but also due to increase in electrolyte (membrane+buffer solution) conductivity. Buffer concentration in the fuel (glucose) solution was found as an important parameter. Under optimum fuel cell operation conditions (i. e. 30–40 °C, pH 5 0.3 M buffer solution), maximum current densities of 3.0–3.2 mA cm⁻² were reached. Low‐power devices (i. e. a calculator, step motor) were powered with 5‐cell stack producing 3 mW at 1.3 V.

中文翻译：

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电化学阻抗谱法评估基于葡萄糖氧化酶的酶燃料电池与新开发的壳聚糖膜的集​​成

通过使用基于壳聚糖的膜以及附着有葡萄糖氧化酶的生物阳极，制备了相当稳定的酶燃料电池（单电池和5电池堆）。在短路条件下监测燃料电池的连续运行，并在一周内达到半衰期。通过电化学阻抗谱（EIS）对pH，温度，缓冲液类型和浓度对不同类型的内部生产的壳聚糖膜的影响进行了详细分析。EIS被用来观察整体电解质电阻，电荷转移特性，质量转移和双层效应对集成燃料电池（单电池和5电池堆）的影响。还通过极化实验分析了燃料电池的性能。燃料电池的电流密度在较高的工作温度下增加，这不仅是由于更好的酶动力学，而且还由于电解质（膜+缓冲溶液）电导率的增加。发现燃料（葡萄糖）溶液中的缓冲液浓度是重要的参数。在最佳燃料电池运行条件下（例如30–40°C，pH 5 0.3 M缓冲溶液），最大电流密度为3.0–3.2 mA cm达到^-2。低功耗设备（即计算器，步进电机）由5单元电池组供电，在1.3 V电压下产生3 mW的功率。