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Organization of the Escherichia coli Chromosome by a MukBEF Axial Core.
Molecular Cell ( IF 16.0 ) Pub Date : 2020-02-24 , DOI: 10.1016/j.molcel.2020.02.003
Jarno Mäkelä 1 , David J Sherratt 1
Affiliation  

Structural maintenance of chromosomes (SMC) complexes organize chromosomes ubiquitously, thereby contributing to their faithful segregation. We demonstrate that under conditions of increased chromosome occupancy of the Escherichia coli SMC complex, MukBEF, the chromosome is organized as a series of loops around a thin (<130 nm) MukBEF axial core, whose length is ∼1,100 times shorter than the chromosomal DNA. The linear order of chromosomal loci is maintained in the axial cores, whose formation requires MukBEF ATP hydrolysis. Axial core structure in non-replicating chromosomes is predominantly linear (1 μm) but becomes circular (1.5 μm) in the absence of MatP because of its failure to displace MukBEF from the 800 kbp replication termination region (ter). Displacement of MukBEF from ter by MatP in wild-type cells directs MukBEF colocalization with the replication origin. We conclude that MukBEF individualizes and compacts the chromosome lengthwise, demonstrating a chromosome organization mechanism similar to condensin in mitotic chromosome formation.

中文翻译:

通过 MukBEF 轴向核心组织大肠杆菌染色体。

染色体结构维持 (SMC) 复合物无处不在地组织染色体,从而有助于它们的忠实分离。我们证明,在大肠杆菌 SMC 复合体 MukBEF 的染色体占有率增加的条件下,染色体被组织成一系列围绕薄(<130 nm)MukBEF 轴核的环,其长度比染色体 DNA 短约 1,100 倍. 染色体基因座的线性顺序保持在轴向核心中,其形成需要 MukBEF ATP 水解。非复制染色体中的轴向核心结构主要是线性的(1 μm),但在没有 MatP 的情况下变为圆形(1.5 μm),因为它无法从 800 kbp 复制终止区域(ter)中取代 MukBEF。在野生型细胞中,MukBEF 从 ter 中被 MatP 置换,可指导 MukBEF 与复制起点的共定位。我们得出结论,MukBEF 纵向个体化和压缩染色体,证明染色体组织机制类似于有丝分裂染色体形成中的凝聚素。
更新日期:2020-02-24
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