Bud dormancy is indispensable for the survival of perennial plants in cold winters. Abscisic acid (ABA) has essential functions influencing the endo‐dormancy status. Dormancy‐associated MADS ‐box /SHORT VEGETATIVE PHASE ‐like genes function downstream of the ABA signalling pathway to regulate bud dormancy. However, the regulation of DAM /SVP expression remains largely uncharacterized. In this study, we confirmed that endo‐dormancy maintenance and PpyDAM3 expression are controlled by the ABA content in pear (Pyrus pyrifolia ) buds. The expression of pear ABRE‐BINDING FACTOR3 (PpyABF3 ) was positively correlated with PpyDAM3 expression. Furthermore, PpyABF3 directly bound to the second ABRE in the PpyDAM3 promoter to activate its expression. Interestingly, both PpyABF3 and PpyDAM3 repressed the cell division and growth of transgenic pear calli. Another ABA‐induced ABF protein, PpyABF2, physically interacted with PpyABF3 and disrupted the activation of the PpyDAM3 promoter by PpyABF3, indicating DAM expression was precisely controlled. Additionally, our results suggested that the differences in the PpyDAM3 promoter in two pear cultivars might be responsible for the diversity in the chilling requirements. In summary, our data clarify the finely tuned regulatory mechanism underlying the effect of ABA on DAM gene expression and provide new insights into ABA‐related bud dormancy regulation.

中文翻译：

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ABA响应性ABRE-BINDING FACTOR3激活DAM3表达以促进亚洲梨的芽休眠。

芽休眠对于多年生植物在寒冷冬季的生存是必不可少的。脱落酸（ABA）具有影响内休眠状态的基本功能。休眠相关MADS -框/ SHORT VEGETATIVE PHASE -样基因功能ABA信号转导途径来调节芽休眠的下游。但是，DAM / SVP表达的调控仍未明确。在这项研究中，我们确认了内休眠维持和PpyDAM3表达受梨（梨梨）芽中ABA含量的控制。梨ABRE-BINDING FACTOR3（PpyABF3的表达）与PpyDAM3表达呈正相关。此外，PpyABF3直接与PpyDAM3启动子中的第二个ABRE结合以激活其表达。有趣的是，PpyABF3和PpyDAM3都抑制了转基因梨愈伤组织的细胞分裂和生长。另一个由ABA诱导的ABF蛋白PpyABF2与PpyABF3发生物理相互作用，并破坏了PpyABF3对PpyDAM3启动子的激活，表明DAM的表达受到了精确控制。此外，我们的结果表明PpyDAM3中的差异两个梨品种中的启动子可能是造成冷藏需求差异的原因。总而言之，我们的数据阐明了ABA对DAM基因表达的影响的微调调控机制，并为ABA相关芽休眠调控提供了新见解。