Secondary caries often occurs due to acids produced by biofilms at the tooth-restoration bonded interface, and is considered as one of the primary reasons for dental restoration failure. The objective of this study was (1) to synthesize nanosized EMT zeolites with silver ion exchanging, (2) to incorporate them into a commercial dental adhesive and (3) investigate the inhibition of biofilm formation at the tooth-restoration margin. The template-free zeolite nanocrystals are synthesized and stabilized in water suspensions and directly used for silver ion-exchange. Streptococcus mutans, Streptococcus gordonii, Streptococcus sanguinis were selected as representative strains of cariogenic pathogens and to form single-species biofilms. Colony-forming units (CFU), live/dead and metabolic activity were measured. The Ag⁺-EMT zeolites addition would not compromise the color and esthetics of dental restoration. EMT zeolites with longest exchanging time (40 min) in the adhesives achieved the greatest reduction in early attachment, bacterial biomass, biofilm growth and metabolic activity. Biofilm CFU counts were reduced by nearly 2 orders of magnitude for all three cariogenic pathogens. Therefore, the adhesives containing Ag⁺ exchanged zeolites had remarkable antibacterial properties to serve as “bioactive” adhesive materials and revealed its potential value for anti-biofilm and anti-caries clinical applications.

继发龋齿通常是由于生物膜在牙齿修复粘结界面处产生的酸而发生的，被认为是造成牙齿修复失败的主要原因之一。这项研究的目的是（1）合成具有银离子交换作用的纳米级EMT沸石，（2）将其掺入市售牙科粘合剂中，以及（3）研究在牙齿修复边缘对生物膜形成的抑制作用。合成无模板的沸石纳米晶体，并使其在水悬浮液中稳定下来，可直接用于银离子交换。变形链球菌，链球菌戈登，血链球菌选择被选为致龋性病原体的代表性菌株并形成单一物种生物膜。测量菌落形成单位（CFU），活/死和代谢活性。Ag ⁺ -EMT沸石的加入不会损害牙齿修复体的颜色和美观。在粘合剂中具有最长交换时间（40分钟）的EMT沸石在早期附着，细菌生物量，生物膜生长和代谢活性方面的降低最大。对于所有三种致癌病原体，生物膜CFU计数均降低了近2个数量级。因此，含有Ag ⁺交换沸石的胶粘剂具有出色的抗菌性能，可以用作“生物活性”胶粘剂材料，并显示出其在抗生物膜和防龋临床应用中的潜在价值。