当前位置: X-MOL 学术Cell. Signal. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
CRISPR system: Discovery, development and off-target detection.
Cellular Signalling ( IF 4.8 ) Pub Date : 2020-02-22 , DOI: 10.1016/j.cellsig.2020.109577
Shengmiao Chen 1 , Yufeng Yao 1 , Yanchun Zhang 1 , Gaofeng Fan 1
Affiliation  

As a revolutionary gene editing tool based on the adaptive immune defense mechanism of bacteria and archaea against exogenous DNA invasion, CRISPR/Cas system shows many remarkable characteristics over ZFNs and TALENs. However, off-target effect remains as one of the major imperfection of CRISPR/Cas system, hindering its further application in translational research. In this review, we highlight major breakthrough cross the development/application of this powerful toolkit, and summarize feasible methods for detecting potential off-target effects during genetic manipulation. We hope this review will assist scientists for accurate genomic editing in their future research.

中文翻译:

CRISPR 系统:发现、开发和脱靶检测。

作为一种革命性的基因编辑工具,基于细菌和古细菌对外源 DNA 入侵的适应性免疫防御机制,CRISPR/Cas 系统显示出许多优于 ZFNs 和 TALENs 的显着特征。然而,脱靶效应仍然是 CRISPR/Cas 系统的主要缺陷之一,阻碍了其在转化研究中的进一步应用。在这篇综述中,我们强调了这个强大工具包的开发/应用的重大突破,并总结了检测基因操作过程中潜在脱靶效应的可行方法。我们希望这篇综述能帮助科学家在他们未来的研究中进行准确的基因组编辑。
更新日期:2020-02-23
down
wechat
bug