BACKGROUND Acute pancreatitis (AP) is a sudden inflammation of the pancreas that may be life-threatening disease with high mortality rates, particularly in the presence of systemic inflammatory response and multiple organ failure. Oxidative stress has been shown to be involved in the pathophysiology of acute pancreatitis. AIM This study is designed to investigate the possible effect of mesna on an experimental model of cerulein-induced acute pancreatitis. METHODS Animals were divided into five groups: Group 1 served as a control group given the saline; group II (mesna group) received mesna at a dose of (100 mg/kg per dose, i.p.) four times; group III (acute pancreatitis group) received cerulein at a dose of (20 µg/kg/dose, s.c.) four times with 1-h intervals; group VI, cerulein + mesna, was treated with mesna at a dose of (100 mg/kg, i.p.) 15 min before each cerulein injection. RESULTS Animals with acute pancreatitis showed elevated serum amylase and lipase levels. Biochemical parameters showed increased pancreatic tumor necrosis factors-α (TNF-α) and interleukin-1β (IL-1β) levels. A disturbance in oxidative stress markers was evident by elevated pancreatic lipid peroxides (TBARS) and decline in pancreatic antioxidants' concentrations including reduced glutathione (GSH); superoxide dismutase (SOD); and glutathione peroxidase (GSH-Px). Histological examination confirmed pancreatic injury. Pre-treatment with mesna was able to abolish the changes in pancreatic enzymes, oxidative stress markers (TBARS, SOD, GSH and GSH-Px), pancreatic inflammatory markers (TNF-α, IL-1β) as well as histological changes. CONCLUSIONS Mesna mitigates AP by alleviating pancreatic oxidative stress damage and inhibiting inflammation.

中文翻译：

---

梅斯纳通过抑制实验大鼠的炎症反应和氧化应激缓解了青霉素诱导的急性胰腺炎。

背景技术急性胰腺炎（AP）是胰腺的突然炎症，其可能是威胁生命的疾病，死亡率很高，尤其是在存在全身性炎症反应和多器官衰竭的情况下。氧化应激已被证明与急性胰腺炎的病理生理有关。目的本研究旨在研究梅斯纳对小脑素诱导的急性胰腺炎实验模型的可能作用。方法将动物分为五组：第一组为对照组，给予生理盐水；第二组为生理盐水。II组（mesna组）以（100mg / kg每剂量，ip）剂量接受mesna四次；第三组（急性胰腺炎组）以1h的间隔四次（20 µg / kg /剂量，皮下注射）接受青霉素。第六组cerulein + mesna接受（100 mg / kg，ip ）每次注射青霉素15分钟前。结果患有急性胰腺炎的动物血清淀粉酶和脂肪酶水平升高。生化指标显示胰腺肿瘤坏死因子-α（TNF-α）和白介素-1β（IL-1β）水平升高。升高的胰腺脂质过氧化物（TBARS）和降低的胰腺抗氧化剂浓度（包括减少的谷胱甘肽（GSH））明显证明了氧化应激标志物的紊乱。超氧化物歧化酶（SOD）; 和谷胱甘肽过氧化物酶（GSH-Px）。组织学检查证实为胰腺损伤。梅斯纳预处理能够消除胰腺酶，氧化应激指标（TBARS，SOD，GSH和GSH-Px），胰腺炎性指标（TNF-α，IL-1β）的变化以及组织学变化。