Burns and chronic wounds are especially challenging wounds to heal. In efforts to heal these wounds, physicians often use autologous skin grafts to help restore mechanical and barrier functionality to the wound area. These grafts are, by nature, limited in availability. In an effort to provide an alternative, we have developed an electrospun wound dressing designed to incorporate into the wound with the option to deliver a cellular payload. Here, a blend of poly(glycolic acid) and poly(ethylene glycol) was electrospun as part of a custom fabrication method that incorporated 3D printed poly(vinyl alcohol) sacrificial elements. This preparation is unique compared to traditional electrospinning as sacrificial elements provide an internal void space for an injectable payload to be delivered to the wound site. When the construct was tested in vivo (full thickness excisional skin wounds), wound closure was slightly delayed by the presence of the scaffold in both normal and challenged wounds. Quality of healing was improved in normal wounds as measured by histomorphometrics when treated with the construct and exhibited increased neovascularization. Our results demonstrate that the extracellular matrix‐like scaffold developed in this study is beneficial to healing of full thickness skin defects and may benefit challenged wounds.

中文翻译：

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用于皮肤伤口愈合的静电纺丝和 3D 打印细胞递送装置的体内评估。

烧伤和慢性伤口尤其难以愈合。为了治愈这些伤口，医生经常使用自体皮肤移植来帮助恢复伤口区域的机械和屏障功能。这些移植物本质上是有限的。为了提供替代方案，我们开发了一种电纺伤口敷料，旨在融入伤口并可选择提供细胞有效载荷。在这里，聚（乙醇酸）和聚（乙二醇）的混合物作为定制制造方法的一部分进行电纺，该方法结合了 3D 打印的聚（乙烯醇）牺牲元素。与传统的静电纺丝相比，这种制备是独一无二的，因为牺牲元件为可注射的有效载荷提供了一个内部空隙空间，以便将其输送到伤口部位。当在体内测试构建体时（全层切除皮肤伤口），正常伤口和受攻击伤口中支架的存在都会略微延迟伤口闭合。当用该构建体治疗时，正常伤口的愈合质量得到改善，如通过组织形态计量学测量的，并表现出增加的新血管形成。我们的结果表明，本研究中开发的细胞外基质样支架有利于全层皮肤缺损的愈合，并可能有益于受到挑战的伤口。