For the last two decades there has been wide ranging debate about the status of macroautophagy during mitosis. Because metazoan cells undergo an "open" mitosis in which the nuclear envelope breaks down, it has been proposed that macroautophagy must be inhibited to maintain genome integrity. While many studies have agreed that the number of autophagosomes is greatly reduced in cells undergoing mitosis, there has been no consensus on whether this reflects decreased autophagosome synthesis or increased autophagosome degradation. Reviewing the literature we were concerned that many studies relied too heavily on autophagy assays that were simply not appropriate for a relatively brief event such as mitosis. Using highly dynamic omegasome markers we have recently shown unequivocally that autophagosome synthesis is repressed at the onset of mitosis and is restored once cell division is complete. This is accomplished by CDK1, the master regulator of mitosis, taking over the function of MTORC1, to ensure autophagy is repressed during mitosis.

中文翻译：

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巨噬细胞在有丝分裂期间被抑制-眼见为实。

在过去的二十年中，关于有丝分裂期间巨噬细胞自噬状态的争论一直很广泛。由于后生动物细胞经历了“开放”的有丝分裂，其中核被膜被破坏，因此提出必须抑制巨噬自噬以维持基因组完整性。尽管许多研究已经同意，经历有丝分裂的细胞中自噬体的数量大大减少，但关于这是否反映了自噬体合成的减少或自噬体降解的增加，尚无共识。回顾文献，我们担心许多研究过于依赖自噬测定法，而这些测定法根本不适用于诸如有丝分裂等相对短暂的事件。最近，使用高度动态的ω-体标记，我们明确表明自噬体的合成在有丝分裂开始时受到抑制，一旦细胞分裂完成，其恢复。这是通过有丝分裂的主要调节剂CDK1接管MTORC1的功能来确保有丝分裂期间自噬被抑制的。