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Histone H3K27 acetylation is dispensable for enhancer activity in mouse embryonic stem cells
Genome Biology ( IF 12.3 ) Pub Date : 2020-02-21 , DOI: 10.1186/s13059-020-01957-w
Tiantian Zhang 1, 2 , Zhuqiang Zhang 1 , Qiang Dong 1 , Jun Xiong 1 , Bing Zhu 1, 2
Affiliation  

H3K27ac is well recognized as a marker for active enhancers and a great indicator of enhancer activity. However, its functional impact on transcription has not been characterized. By substituting lysine 27 in histone variant H3.3 with arginine in mouse embryonic stem cells, we diminish the vast majority of H3K27ac at enhancers. However, the transcriptome is largely undisturbed in these mutant cells, likely because the other enhancer features remain largely unchanged, including chromatin accessibility, H3K4me1, and histone acetylation at other lysine residues. Our results clearly reveal that H3K27ac alone is not capable of functionally determining enhancer activity.

中文翻译:

组蛋白 H3K27 乙酰化对于小鼠胚胎干细胞中的增强子活性是不必要的

H3K27ac 被公认为活性增强子的标记物和增强子活性的重要指标。然而,其对转录的功能影响尚未得到表征。通过在小鼠胚胎干细胞中用精氨酸替换组蛋白变体 H3.3 中的赖氨酸 27,我们减少了增强子中的绝大多数 H3K27ac。然而,转录组在这些突变细胞中基本未受干扰,这可能是因为其他增强子特征基本保持不变,包括染色质可及性、H3K4me1 和其他赖氨酸残基处的组蛋白乙酰化。我们的结果清楚地表明,单独的 H3K27ac 不能在功能上确定增强子的活性。
更新日期:2020-02-21
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