We delineated the expression of DAP12 (DNAX-Activating Protein) and its associated receptors, TREM-1, TREM-2 and MDL-1 in pig alveolar monocyte/macrophages (AMM) that have attained M1 or M2 phenotypes. Pig AMM stimulated in vitro with IFN-γ and IL-4 induced the expression of M1 (TNFα and iNOS) and M2 (ARG1 and no MMR) phenotypic markers, respectively. In influenza virus infected pigs at seven days post-infection, in addition to substantial modulations in the M1 and M2 markers expression, DAP12, TREM-1 and MDL-1 were downregulated in AMM. Thus, DAP12 signaling promoted the anti-inflammatory pathway in AMM of influenza virus infected pigs.

中文翻译：

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猪对流感病毒感染的肺部炎症反应受DAP12和巨噬细胞M1和M2表型的调节。

我们描述了DAP12（DNAX激活蛋白）及其相关受体TREM-1，TREM-2和MDL-1在已达到M1或M2表型的猪肺泡单核细胞/巨噬细胞（AMM）中的表达。IFN-γ和IL-4体外刺激的猪AMM分别诱导M1（TNFα和iNOS）和M2（ARG1和无MMR）表型标记的表达。在感染后7天感染流感病毒的猪中，除了M1和M2标志物表达的大量调节外，AMM中的DAP12，TREM-1和MDL-1也被下调。因此，DAP12信号传导促进了流感病毒感染的猪的AMM中的抗炎途径。