Nitric oxide (NO) is an important signaling molecule implicated in numerous physiological and pathological conditions. NO homeostasis is crucial to its proper functions and misregulation of NO production is implicated in a large number of pathologies. The effect of NO depends on the cell microenvironment as well as its concentration, spatial, and temporal constraints. Thus, the visualization and monitoring of NO in living systems would be valuable for understanding the origin, activities, and biological effects of NO. This review focuses on organic small-molecule fluorescent probes for selective detection and imaging of endogenous NO in vitro and in vivo. The progress suggests that fluorescence imaging is a vital and rapidly technology for real-time detection of NO in biological system.

中文翻译：

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一氧化氮检测和生物成像荧光探针的最新进展。

一氧化氮（NO）是一种重要的信号分子，涉及许多生理和病理状况。NO动态平衡对其正常功能至关重要，并且NO产生的调控异常与许多病理学有关。NO的作用取决于细胞的微环境及其浓度，空间和时间限制。因此，活体系统中NO的可视化和监视对于理解NO的起源，活动和生物学效应将非常有价值。这篇综述着重于有机小分子荧光探针，用于在体外和体内选择性检测和成像内源性NO。研究进展表明，荧光成像技术是实时检测生物系统中NO的重要技术。