Agricultural straw is abundant worldwide, but efficient production of lactic acid from straw is still challenging. In this paper, Trichoderma viride R16 was cultivated using corncob as the substrate in a fed‐batch process, after which the enzymes from T. viride R16 broth were analyzed through protein mass spectrometry and used for lactic acid production. Dioxygenase activity was found in the T. viride R16 enzymes, and 0.2 g/L phenolics (vanillin, 4‐hydroxybenzaldehyde, syringaldehyde) was degraded during enzyme production. Lactic acid production and yield from NH₃‐H₂O₂ pretreated and washed corncob were increased by more than 24% and total phenolics was significantly lower using T. viride R16 enzymes compared with commercial enzymes in batch and fed‐batch fermentation. These characteristics of T. viride R16 support industrial applications with efficient in situ detoxification of phenolic inhibitors without adding an extra step during lactic acid production from pretreated agricultural straw using simultaneous saccharification and fermentation. © 2020 Society of Chemical Industry and John Wiley & Sons, Ltd

中文翻译：

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通过同时糖化，生物解毒和发酵提高凝结芽孢杆菌的乳酸生产

农业秸秆在世界范围内非常丰富，但是从秸秆中高效生产乳酸仍然具有挑战性。在本文中，绿色木霉R16是使用玉米芯为在补料分批工艺中，衬底，之后，从酶培养绿色木霉R16肉汤中通过蛋白质质谱分析并用于乳酸的生产。在T. viride R16酶中发现了双加氧酶活性，在酶生产过程中降解了0.2 g / L的酚类（香兰素，4-羟基苯甲醛，丁香醛）。使用NH ₃ -H ₂ O ₂预处理和洗涤的玉米芯，乳酸产量和产率提高了24％以上，总酚含量显着降低，分批发酵和补料分批发酵中的T. viride R16酶与市售酶相比。T. viride R16的这些特性可通过有效的酚类抑制剂原位解毒来支持工业应用，而无需在通过同时糖化和发酵从预处理过的农业秸秆生产乳酸的过程中增加额外的步骤。©2020年化学工业协会和John Wiley＆Sons，Ltd