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Beyond oil degradation: enzymatic potential of Alcanivorax to degrade natural and synthetic polyesters.
Environmental Microbiology ( IF 5.1 ) Pub Date : 2020-02-27 , DOI: 10.1111/1462-2920.14947
Vinko Zadjelovic 1 , Audam Chhun 1 , Mussa Quareshy 1 , Eleonora Silvano 1 , Juan R Hernandez-Fernaud 1, 2 , María M Aguilo-Ferretjans 1, 3 , Rafael Bosch 3, 4 , Cristina Dorador 5, 6, 7 , Matthew I Gibson 8, 9 , Joseph A Christie-Oleza 1, 3, 4
Affiliation  

Pristine marine environments are highly oligotrophic ecosystems populated by well-established specialized microbial communities. Nevertheless, during oil spills, low-abundant hydrocarbonoclastic bacteria bloom and rapidly prevail over the marine microbiota. The genus Alcanivorax is one of the most abundant and well-studied organisms for oil degradation. While highly successful under polluted conditions due to its specialized oil-degrading metabolism, it is unknown how they persist in these environments during pristine conditions. Here, we show that part of the Alcanivorax genus, as well as oils, has an enormous potential for biodegrading aliphatic polyesters thanks to a unique and abundantly secreted alpha/beta hydrolase. The heterologous overexpression of this esterase proved a remarkable ability to hydrolyse both natural and synthetic polyesters. Our findings contribute to (i) better understand the ecology of Alcanivorax in its natural environment, where natural polyesters such as polyhydroxyalkanoates (PHA) are produced by a large fraction of the community and, hence, an accessible source of carbon and energy used by the organism in order to persist, (ii) highlight the potential of Alcanivorax to clear marine environments from polyester materials of anthropogenic origin as well as oils, and (iii) the discovery of a new versatile esterase with a high biotechnological potential.

中文翻译:

除油降解之外:Alcanivorax具有降解天然和合成聚酯的酶促潜力。

原始的海洋环境是高度贫营养的生态系统,由成熟的专业微生物群落组成。然而,在漏油期间,低丰度的碎屑碎屑细菌大量繁殖并迅速在海洋微生物群中盛行。Alcanivorax属是石油降解最丰富,研究最深入的生物之一。尽管由于其专门的油降解代谢而在污染条件下获得了成功,但未知它们在原始条件下如何在这些环境中持续存在。在这里,我们显示出Alcanivorax属的一部分以及油类,由于其独特且分泌丰富的α/β水解酶,具有生物降解脂族聚酯的巨大潜力。该酯酶的异源过表达证明了其水解天然和合成聚酯的显着能力。
更新日期:2020-04-01
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