Magnetic hyperthermia (MHT) is a promising approach for cancer therapy. However, a systematic MHT characterization as function of temperature on the therapeutic efficiency is barely analyzed. Here, we first perform comparative temperature-dependent analysis of the cobalt ferrite nanoparticles-mediated MHT effectiveness in two murine tumors models - breast (4T1) and colon (CT26) cancer in vitro and in vivo. The overall MHT killing capacity in vitro increased with the temperature and CT26 cells were more sensitive than 4T1 when heated to 43 °C. Well in line with the in vitro data, such heating cured non-metastatic CT26 tumors in vivo, while only inhibiting metastatic 4T1 tumor growth without improving the overall survival. High-temperature MHT (>47 °C) resulted in complete 4T1 primary tumor clearance, 25-40% long-term survival rates, and, importantly, more effective prevention of metastasis comparing to surgical extraction. Thus, the specific MHT temperature must be defined for each tumor individually to ensure a successful antitumor therapy.

中文翻译：

---

温度控制的磁性纳米粒子热疗抑制原发性肿瘤生长和转移扩散。

磁热疗（MHT）是一种有前途的癌症治疗方法。然而，很少分析作为温度对治疗效率的函数的系统性MHT表征。在这里，我们首先在体外和体内对两种鼠类肿瘤模型-乳腺癌（4T1）和结肠癌（CT26）进行钴铁氧体纳米颗粒介导的MHT有效性的比较温度依赖性分析。随着温度的升高，体外总体MHT杀伤能力增加，加热到43°C时，CT26细胞比4T1更敏感。此类加热与体外数据非常吻合，此类加热可在体内治愈非转移性CT26肿瘤，而仅抑制转移性4T1肿瘤的生长而不会提高总体生存率。高温MHT（> 47°C）可以完全清除4T1原发性肿瘤，长期存活率为25-40％，并且 重要的是，与手术拔牙相比，更有效地预防了转移。因此，必须分别为每个肿瘤定义特定的MHT温度，以确保成功的抗肿瘤治疗。