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Enhanced Vitamin C Production Mediated by an ABA-Induced PTP-like Nucleotidase Improves Plant Drought Tolerance in Arabidopsis and Maize.
Molecular Plant ( IF 27.5 ) Pub Date : 2020-02-14 , DOI: 10.1016/j.molp.2020.02.005
Hui Zhang 1 , Yanli Xiang 1 , Neng He 1 , Xiangguo Liu 2 , Hongbo Liu 1 , Liping Fang 1 , Fei Zhang 1 , Xiaopeng Sun 1 , Delin Zhang 1 , Xingwang Li 1 , William Terzaghi 3 , Jianbing Yan 1 , Mingqiu Dai 1
Affiliation  

Abscisic acid (ABA) is a key phytohormone that mediates environmental stress responses. Vitamin C, or L-ascorbic acid (AsA), is the most abundant antioxidant protecting against stress damage in plants. How the ABA and AsA signaling pathways interact in stress responses remains elusive. In this study, we characterized the role of a previously unidentified gene, PTPN (PTP-like Nucleotidase) in plant drought tolerance. In Arabidopsis, (AtPTPN was expressed in multiple tissues and upregulated by ABA and drought treatments. Loss-of-function mutants of AtPTPN were hyposensitive to ABA but hypersensitive to drought stresses, whereas plants with enhanced expression of AtPTPN showed opposite phenotypes to . Overexpression of maize PTPN (ZmPTPN) promoted, while knockdown of ZmPTPN inhibited plant drought tolerance, indicating conserved and positive roles of PTPN in plant drought tolerance. We found that both AtPTPN and ZmPTPN release Pi by hydrolyzing GDP/GMP/dGMP/IMP/dIMP, and that AtPTPN positively regulated AsA production via endogenous Pi content control. Consistently, overexpression of VTC2, the rate-limiting synthetic enzyme in AsA biosynthesis, promoted AsA production and plant drought tolerance, and these effects were largely dependent on AtPTPN activity. Furthermore, we demonstrated that the heat shock transcription factor HSFA6a directly binds the AtPTPN promoter and activates AtPTPN expression. Genetic analyses showed that AtPTPN is required for HSFA6a to regulate ABA and drought responses. Taken together, our data indicate that PTPN-mediated crosstalk between the ABA signaling and AsA biosynthesis pathways positively controls plant drought tolerance.



中文翻译:

ABA诱导的PTP样核苷酸酶介导的维生素C产量提高,提高了拟南芥和玉米的植物耐旱性。

脱落酸(ABA)是介导环境胁迫反应的关键植物激素。维生素C或L-抗坏血酸(AsA)是最丰富的抗氧化剂,可保护植物免受压力伤害。ABA和AsA信号通路如何在应激反应中相互作用尚不清楚。在这项研究中,我们表征了以前未鉴定的基因PTPNPTP样核苷酸酶)在植物抗旱性中的作用。在拟南芥中,(AtPTPN 在多种组织中表达,并受ABA和干旱处理上调。AtPTPN的功能丧失型突变体对ABA敏感性低,但对干旱胁迫高度敏感,而AtPTPN表达增强的植物表现出相反的表型。促进了玉米PTPNZmPTPN)的过表达,而敲低ZmPTPN抑制了植物的抗旱性,表明PTPN在植物抗旱性中的保守作用和积极作用。我们发现AtPTPN和ZmPTPN都通过水解GDP / GMP / dGMP / IMP / dIMP释放Pi,并且AtPTPN通过内源性Pi含量控制正调控AsA的生产。一致地,VTC2的过表达,即AsA生物合成中的限速合成酶,促进了AsA的产生和植物的耐旱性,这些作用很大程度上取决于AtPTPN的活性。此外,我们证明了热激转录因子HSFA6a直接结合AtPTPN启动子并激活AtPTPN表达。遗传分析表明,HSFA6a调节ABA和干旱响应需要AtPTPN。两者合计,我们的数据表明ABA信号转导和AsA生物合成途径之间PTPN介导的串扰积极地控制植物的耐旱性。

更新日期:2020-02-14
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