Peripheral blood stem cells (PBSC) have been increasingly used for allogeneic hematopoietic cell transplantation compared to bone marrow stem cells. Currently, the National Marrow Donor Program (NMDP) policy recommends 5 days of daily filgrastim followed by either 1 or 2 days of apheresis for unrelated donors, depending on collection center choice. To date, there are no studies that compare the differences in donor experience between one and two days of apheresis. We examined 22,348 adult unrelated donor collections in 184 centers from 2006-2016. 20,004 (89.5%) donors had collections on 1 day vs. 2,344 (9.5%) over 2 days. Information on why donors were apheresed in one day vs. two days were not available. Donors who underwent apheresis in 1 day were more likely to be male (67% vs. 46%, p<0.001), younger (age <30 years 48% vs. 36%, p<0.001) and have a higher body weight (83.0 kg vs. 75.9 kg, p<0.001) and BMI (BMI>30: 30% vs. 22%, p<0.001). Successful collection of the requested CD34+ cell count was achieved on the first day in 82% of one day collections vs 16% of 2-day collections. Despite not administering filgrastim the evening after the first day of collection in patients who underwent 2 days of apheresis, the median concentration of CD34+ cells/L in the product on the second day of apheresis was higher than the first day (23.8 × 106 CD34+/L 1st day vs. 28.7 × 106 CD34+/L 2nd day, p<0.001). Donors collected in one day were less likely to experience citrate toxicity (36% vs 52%, p<0.001), hospitalizations (1% vs 6%, p<0.001), and other side effects related to apheresis (Modified Toxicity Criteria incidence: 20% vs. 26%, p<0.001). Female sex, older age, collection via central lines, and greater BMI were factors associated with greater likelihood for development of toxicity whereas less toxicity was noted in those with higher CD34+ counts and more blood processed on the first day of collection. We conclude that although unrelated donors can be successfully collected in one or two days, one day apheresis procedures were associated with less overall toxicity and we therefore recommend single day collections, especially if the requested number of cells have been collected in one day.

中文翻译：

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与不相关捐献者的2天相比，捐献者的毒性降低与1天的外周血祖细胞的收集有关。

与骨髓干细胞相比，外周血干细胞（PBSC）已越来越多地用于同种异体造血细胞移植。目前，国家骨髓捐献者计划（NMDP）政策建议非亲缘捐献者每天进行5天非格司亭，然后进行1或2天的单采血，具体取决于收集中心的选择。迄今为止，还没有研究可以比较单采一两天之间供体经验的差异。我们从2006-2016年在184个中心检查了22,348个与成年人无关的成人捐赠者收藏。20,004（89.5％）个捐助者在第1天收到了捐款，而2天有2,344（9.5％）个。没有关于为何在一天和两天之内捐献者被解散的信息。在1天接受单采的捐献者更可能是男性（67％比46％，p <0.001），年轻（年龄<30岁48％vs. 36％，p <0）。001）和更高的体重（83.0公斤vs. 75.9公斤，p <0.001）和BMI（BMI> 30：30％vs. 22％，p <0.001）。在第一天成功收集了请求的CD34 +细胞计数，一天收集的82％，两天收集的16％。尽管接受单采血液分离2天的患者在收集第一天后的晚上未给予非格司亭，但单采血液分离第二天产品中CD34 +细胞/ L的中位数浓度仍高于第一天（23.8×106 CD34 + /第1天L vs.28.7×106 CD34 + / L第2天，p <0.001）。一天内收集到的捐赠者发生柠檬酸盐毒性的可能性较小（36％vs 52％，p <0.001），住院（1％vs 6％，p <0.001）和其他与血液分离术相关的副作用（改良毒性标准发生率： 20％和26％，p <0.001）。女性，年龄较大，通过中心线收集血红蛋白和更大的BMI是产生毒性可能性更大的相关因素，而在CD34 +计数较高且在收集的第一天处理的血液较多的那些中，毒性较小。我们得出的结论是，尽管可以在一两天内成功收集无关的供体，但一天的采血程序与总体毒性较低有关，因此我们建议单日收集，特别是如果一天中已收集了所需数量的细胞。