Single cell RNA-seq reveals the landscape of tumor and infiltrating immune cells in nasopharyngeal cancer.,Cancer Letters

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Single cell RNA-seq reveals the landscape of tumor and infiltrating immune cells in nasopharyngeal cancer.
Cancer Letters ( IF 9.7 ) Pub Date : 2020-02-13 , DOI: 10.1016/j.canlet.2020.02.010
Jin Zhao ₁ , Can Guo ₂ , Fang Xiong ₂ , Jianjun Yu ₃ , Junshang Ge ₂ , Hui Wang ₃ , Qianjin Liao ₃ , Yujuan Zhou ₃ , Qian Gong ₃ , Bo Xiang ₄ , Ming Zhou ₄ , Xiaoling Li ₄ , Guiyuan Li ₄ , Wei Xiong ₁ , Jian Fang ₅ , Zhaoyang Zeng ₁

Affiliation

NHC Key Laboratory of Carcinogenesis and Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China; Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, Hunan, China.
Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, Hunan, China.
NHC Key Laboratory of Carcinogenesis and Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China.
NHC Key Laboratory of Carcinogenesis and Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, China; Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha, Hunan, China; Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, the Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences, Health Science Center, Shenzhen University, Shenzhen, Guangdong, China.

Nasopharyngeal cancer (NPC) is one of the most malignant tumors in Southern China and southeast Asia, which is characterized by a dense lymphocyte infiltration and a poor prognosis. The emergence of single-cell sequencing represents a powerful tool to resolve tumor heterogeneity and delineate the complex communication among the tumor cells with neighboring stromal and immune cells in the tumor microenvironment (TME). Here, we performed single cell RNA-seq and analyzed tumor cells together with the infiltrating immune cells from three NPC tumor tissues. In our study, the malignant cells display the intra- and inter-tumoral heterogeneity among the individual patients. Analysis of the immune cells reveal the heterogeneous composition of the distinct immune cells and the various functional states of T cells in NPC tumors. Additionally, coupled with the reconstruct of the T cell receptor (TCR) sequences from immune cells full-length single-cell sequence data, we identify the diverse T cell clonotypes and expansion distribution in individual tumors. Overall, we firstly reveal the landscape of tumor and infiltrating immune cells in nasopharyngeal cancer. These results provide deeper insights on the mechanisms of tumor clearance by immune cells in the surrounding microenvironment, which will be helpful in improving the targeted and immune therapies for NPC.

更新日期：2020-02-20

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