The standard approach to detect misuse with testosterone in sport is based on the determination and evaluation of the urinary steroid profile followed by the confirmation of atypical profiles using isotope ratio mass spectrometry. The detection capacity of these methods can be attenuated by confounding factors or testosterone preparations with endogenous isotopic fingerprints. An alternative detection method for misuse of an endogenous steroid in sports is the direct detection of the administered steroid ester present in most preparations. Thus unambiguous proof for doping misuse can be delivered. In this work, the sensitivity of gas chromatography coupled to a triple quadrupole with chemical ionization (GC-CI-MS/MS) is applied to detect trace levels of 10 testosterone and 2 nandrolone esters in plasma for in human doping analysis. The detection method was developed employing a liquid-liquid extraction and HPLC cleanup step before analysis on the GC-CI-MS/MS. The quantitative method was validated in a linear range of 100-2000 pg/ml and proved to be selective, reproducible and very sensitive with limits of detection as low as to 10 pg/ml. A clinical study with the administration of testosterone undecanoate in 3 volunteers was carried out and the compound was detectable up to 86 days after administration.

中文翻译：

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验证了使用正化学电离GC-MS / MS对血浆中类固醇酯进行掺杂分析的超灵敏检测方法的有效性。

检测运动中睾丸激素滥用的标准方法是基于对尿类固醇激素谱的测定和评估，然后使用同位素比质谱法确定非典型谱。这些方法的检测能力可能会由于混杂因素或具有内源性同位素指纹的睾丸激素制剂而减弱。一种在体育运动中滥用内源性类固醇的替代检测方法是直接检测大多数制剂中存在的已施用类固醇酯。因此，可以为掺杂滥用提供明确的证明。在这项工作中，将气相色谱法与化学电离的三重四极杆耦合的灵敏度（GC-CI-MS / MS）用于检测血浆中痕量水平的10种睾丸激素和2种nandrolone酯，以进行人体掺杂分析。在GC-CI-MS / MS上进行分析之前，采用液-液萃取和HPLC净化步骤开发了检测方法。定量方法在100-2000 pg / ml的线性范围内得到验证，并被证明具有选择性，可重复性且非常灵敏，检测限低至10 pg / ml。在3名志愿者中进行了十一酸睾丸激素给药的临床研究，该化合物在给药后长达86天可被检测到。