当前位置：
X-MOL 学术
›
J. Chromatogr. B
›
论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Discovery and validation of biomarkers for Zhongning goji berries using liquid chromatography mass spectrometry.
Journal of Chromatography B ( IF 3 ) Pub Date : 2020-02-17 , DOI: 10.1016/j.jchromb.2020.122037 Wei Lv 1 , Nan Zhao 2 , Qiang Zhao 3 , Shuai Huang 3 , Dan Liu 3 , Zhenyu Wang 4 , Jin Yang 5 , Xiaozhe Zhang 3
Journal of Chromatography B ( IF 3 ) Pub Date : 2020-02-17 , DOI: 10.1016/j.jchromb.2020.122037 Wei Lv 1 , Nan Zhao 2 , Qiang Zhao 3 , Shuai Huang 3 , Dan Liu 3 , Zhenyu Wang 4 , Jin Yang 5 , Xiaozhe Zhang 3
Affiliation
|
Daodi medicinal material (DMM), which is traditional Chinese herbal medicine that has been used for long periods and have gained credibility in clinical practice, is part of the Chinese culture. However, Zhongning Goji berries (ZNG), a DMM, are illegally adulterated in the market by adding non Zhongning goji berries (NZNG). Consequently, the development of biomarker(s) is necessary for proper identification of ZNG and NZNG. In this study, a nontargeted metabolomics approach based on ultra high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) was used to find the differential composition between ZNG and NZNG. Using a combination of single-factor and multivariate statistical analyses, seven compounds with significant differences were discovered and identified, one of which was an unreported compound (a glycoside of pyrrolidine alkaloid). These compounds could be used as single biomarkers for receiver operating characteristic (ROC) analysis. In particular, the binary logistic regression result showed that two sets of combinative biomarkers to distinguish ZNG from NZNG with good sensitivity and specificity. Moreover, there was a significant positive correlation between the two combinative biomarkers and the glycoside of pyrrolidine alkaloid. The results of this study provide new ideas on the developments of ZNG identification, authenticity control and against adulteration in the Chinese circulation market.
更新日期：2020-02-20
京公网安备 11010802027423号