The level of chloride ions (Cl⁻) in sweat is a recognized biomarker for the genetic disorder cystic fibrosis (CF). The accurate quantitation of chloride ions in sweat is therefore a vital diagnostic tool for this life-threatening disease. In this work, a fluorescent, chloride ion chemosensor was developed by exploiting the strong interaction between silver (Ag⁺) and chloride ions. Using the concept of ligand displacement, six Ag⁺-benzimidazole complexes were prepared as candidate chloride ion chemosensors. The Ag⁺-2-(Furan-2-yl)-1H-benzo[d]imidazole (Ag⁺-FBI) complex was identified as the optimal sensor owing to its Ag⁺-FBI high sensitivity (limit of detection = 19 μM), short response time (<3 min), and remarkable fluorescence turn-on response across a broad pH range (pH 6–9). The Ag⁺-FBI complex exhibited high selectivity for Cl⁻ ions and was successfully used to quantify chloride ions in artificial sweat samples containing multiple ions and other biological constituents. This unique, simple, and effective probe thus shows great potential for clinical diagnostic applications.

汗液中的氯离子（Cl +）水平是遗传性疾病囊性纤维化（CF）的公认生物标志物。因此，对汗液中氯离子的准确定量是这种威胁生命的疾病的重要诊断工具。在这项工作中，通过利用银（Ag ⁺）与氯离子之间的强相互作用，开发了一种荧光的氯离子化学传感器。使用配体置换的概念，制备了六个Ag ^+-苯并咪唑配合物作为候选氯离子化学传感器。银⁺ -2-（呋喃-2-基）-1- ħ -苯并[ d ]咪唑（银⁺ - FBI）络合物被鉴定为由于其银的最佳传感器⁺ -FBI灵敏度高（检测极限= 19μM），响应时间短（<3分钟），在宽pH范围（pH 6–9）中均具有显着的荧光开启响应。银⁺ - FBI复杂表现出高的对的Cl -离子和选择性被成功地用于含有多个离子和其它生物成分人工汗液样品中进行量化氯离子。因此，这种独特，简单而有效的探针在临床诊断应用中显示出巨大的潜力。