It is very important to determine mercury owing to its complex biological poisonousness that could cause various physical diseases and even death. In this study, we designed a novel coumarin-based ratiometric fluorescent probe, CR-Hg, with DL-dithiothreitol moiety as the recognition receptor to detect mercury ions (Hg²⁺). CR-Hg could realize the quantitative detection of Hg²⁺ between 0 and 1.4 μM with low detection limit (1.6 nM). Furthermore, CR-Hg could react quickly with Hg²⁺ in a few seconds and accompanied by color changes from pale yellow to orange. In the end, the applications of CR-Hg in the water samples, living cells and zebrafish more deeply indicated that the probe could serve as a novel promising tool for revealing mercury distribution in the environment and organisms.

由于汞具有复杂的生物毒性，可能导致各种物理疾病甚至死亡，因此确定汞非常重要。在这项研究中，我们设计了一种新颖的基于香豆素的比例荧光探针CR-Hg，其中DL-二硫苏糖醇部分作为识别受体来检测汞离子（Hg ²⁺）。CR-Hg可以定量检测0至^1.4μM之间的Hg ²⁺，检测限低（1.6 nM）。此外，CR-Hg可以在几秒钟内与Hg ²⁺快速反应，并伴有从浅黄色到橙色的颜色变化。最后，CR-Hg的应用 在水样中，活细胞和斑马鱼更深地表明，该探针可以作为揭示环境和生物中汞分布的有前途的新工具。