OBJECTIVE To investigate the role of miR-411-5p and miR-434-3p in osteoblast differentiation in particulate-induced osteolysis. METHODS A mouse model of osteolysis and an in vitro osteolysis model were constructed. The expressions of molecules were detected using qRT-PCR and western blot. Alkaline phosphatase (ALP) activity was measured using the ALP Assay Kit, and the bone mineralization was measured using alizarin red staining. RESULTS The expression of miR-411-5p and miR-434-3p was decreased in osteolysis mice and UHMWPE-induced mMSCs, while GATA4 protein expression was increased. Over-expression of miR-411-5p and miR-434-3p up-regulated the expressions of osteoblast gene markers, enhanced the ALP activity, promoted the bone mineralization of mesenchymal stem cells. In addition, miR-411-5p and miR-434-3p could target GATA4, and miR-411-5p/434-3p affected the expressions of osteoblast gene markers through GATA4 in vitro and in vivo. CONCLUSION Overexpression of miR-411-5p and miR-434-3p promoted the osteoblast differentiation by inhibiting GATA4 expression.

中文翻译：

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miR-411-5p和miR-434-3p的过表达通过靶向GATA4促进成骨细胞分化。

目的探讨miR-411-5p和miR-434-3p在颗粒诱导的骨溶解中在成骨细胞分化中的作用。方法建立小鼠骨溶解模型和体外骨溶解模型。使用qRT-PCR和western blot检测分子的表达。使用ALP分析试剂盒测量碱性磷酸酶（ALP）活性，并使用茜素红染色测量骨矿化程度。结果在溶骨小鼠和UHMWPE诱导的mMSC中，miR-411-5p和miR-434-3p的表达降低，而GATA4蛋白的表达增加。miR-411-5p和miR-434-3p的过表达上调了成骨细胞基因标志物的表达，增强了ALP活性，促进了间充质干细胞的骨矿化。此外，miR-411-5p和miR-434-3p可以靶向GATA4，miR-411-5p / 434-3p通过体内外GATA4影响成骨细胞基因标志物的表达。结论miR-411-5p和miR-434-3p的过表达通过抑制GATA4的表达促进成骨细胞的分化。