OBJECTIVE To evaluate, by means of an ex-vivo human tooth-culture model and in-vivo minipig animal study, the pulpal inflammatory reaction and reparative dentin-formation capacity of an injectable phosphopullulan-based calcium-silicate cement (GC, Tokyo, Japan) upon pulp capping, this in comparison with the commercial reference material Biodentine (Septodont). METHODS For the ex-vivo tooth model, 9 freshly-extracted teeth from 3 different patients were pulp-capped with the experimental biomaterial (n = 3), Biodentine (n = 3) or left uncapped (control; n = 3). The teeth were kept in fresh culture medium for 4 weeks and, upon fixation three-dimensional Micro-CT and histology were performed. For the in-vivo animal study, 40 teeth from 3 minipigs were exposed and pulp capped with the experimental biomaterial containing phosphopullulan (n = 24) or Biodentine (n = 16) for 7 or 70 days. The inflammatory reaction and the tissue-regenerative potential was qualitatively and semi-quantitatively characterized using three-dimensional micro-CT and histology. RESULTS Ex vivo, the treatment with the experimental phosphopullulan-based calcium-silicate cement and Biodentine stimulated the formation of fibrous tissue and mineralized foci. In vivo, early inflammatory reaction and regeneration of the pulp-tissue interface was promoted by both bioceramic materials after 7 and 70 days, respectively. SIGNIFICANCE Our findings bring new insights into calcium-silicate-mediated dental pulp repair and regeneration. The novel ready-to-use and self-adhering functionalized calcium-silicate cement revealed effective pulpal repair potential.

中文翻译：

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用于纸浆组织工程的可注射磷酸普卢兰官能化硅酸钙水泥：一项体内和体外研究。

目的通过离体人类牙齿培养模型和体内小型猪动物研究评估可注射磷普鲁兰基硅酸钙水泥的牙髓炎症反应和修复性牙本质形成能力（GC，东京，日本） ）进行纸浆加盖后，与商业参考材料Biodentine（Septodont）相比。方法对于离体牙齿模型，将来自3位不同患者的9颗新鲜提取的牙齿用实验生物材料（n = 3），Biodentine（n = 3）进行牙髓封闭或不封盖（对照组； n = 3）。将牙齿在新鲜的培养基中放置4周，固定后进行三维Micro-CT和组织学检查。对于体内动物研究，暴露来自3只小型猪的40颗牙齿，并用含磷普卢兰（n = 24）或Biodentine（n = 16）的实验生物材料将牙髓覆盖7或70天。使用三维显微CT和组织学对炎症反应和组织再生潜力进行定性和半定量表征。结果离体后，用基于磷普鲁兰的实验性硅酸钙水泥和Biodentine的处理刺激了纤维组织和矿化灶的形成。在体内，两种生物陶瓷材料分别在7天和70天后促进早期炎症反应和牙髓-组织界面的再生。意义我们的发现为硅酸钙介导的牙髓修复和再生带来了新的见解。