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Analysis of two Mexican Pectobacterium brasiliense strains reveals an inverted relationship between c-di-GMP levels with exopolysaccharide production and swarming motility.
Microbiological Research ( IF 6.7 ) Pub Date : 2020-02-19 , DOI: 10.1016/j.micres.2020.126427
Delia A Narváez-Barragán 1 , Andrés de Sandozequi 1 , Mabel Rodríguez 1 , Karel Estrada 2 , Omar E Tovar-Herrera 1 , Claudia Martínez-Anaya 1
Affiliation  

Pectobacterium is a diverse genus of phytopathogenic species from soil and water that cause infection either to restricted or multiple plant hosts. Phylogenetic analysis and metabolic fingerprinting of large numbers of genomes have expanded classification of Pectobacterium members. Pectobacterium brasiliense sp. nov has been elevated to the species level having detached from P. carotovorum. Here we present two P. brasiliense strains BF20 and BF45 isolated in Mexico from Opuntia and tobacco, respectively, which cluster into two different groups in whole genome comparisons with other Pectobacterium. We found that BF20 and BF45 strains are phenotypically different as BF45 showed more severe and rapid symptoms in comparison to BF20 in the host models celery and broccoli. Both strains produced similar levels of the main autoinducers, but BF45 shows an additional low abundant autoinducer compared to strain BF20. The two strains had different levels of c-di-GMP, which regulates the transition from motile to sessile lifestyle. In contrast to BF45, BF20 had the highest levels of c-di-GMP, was more motile (swarming), non-flocculant and less proficient in biofilm formation and exopolysaccharide production. Genomic comparisons revealed that differences in c-di-GMP accumulation and perhaps the associated phenotypes might be due to unique c-di-GMP metabolic genes in these two strains. Our results improve our understanding of the associations between phenotype and genotype and how this has shaped the physiology of Pectobacterium strains.



中文翻译:

对两种墨西哥巴西油酸杆菌菌株的分析显示,c-di-GMP水平与胞外多糖产生和成群运动之间存在反向关系。

芽孢杆菌是土壤和水中的植物致病菌的多种属,可导致受限制或多种植物宿主感染。系统发育分析和代谢指纹图谱的大量基因组已经扩展了对分枝杆菌成员的分类。黑腐brasiliense SP。nov已被升高到与胡萝卜假单胞菌分离的物种水平。在这里,我们介绍了分别从墨西哥的仙人掌和烟草中分离出的两个巴西假单胞菌菌株BF20和BF45 ,它们在与其他果胶杆菌的全基因组比较中均分为两个不同的组。我们发现BF20和BF45菌株在表型上存在差异,因为在宿主模型芹菜和西兰花中,与BF20相比,BF45显示出更为严重和快速的症状。两种菌株产生的主要自诱导物水平相似,但与菌株BF20相比,BF45表现出另外的低丰度自诱导物。两种菌株具有不同水平的c-di-GMP,可调节从运动型向无柄型生活方式的转变。与BF45相比,BF20的c-di-GMP含量最高,运动性强(成群),不絮凝,生物膜形成和胞外多糖生产能力较差。基因组比较显示,c-di-GMP积累和相关表型的差异可能是由于这两个菌株中独特的c-di-GMP代谢基因所致。果胶菌株。

更新日期:2020-02-19
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