当前位置: X-MOL 学术Mol. Immunol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Oral intervention of Lactobacillus pentosus S-PT84 attenuates the allergenic responses in a BALB/C mouse model of egg allergy.
Molecular Immunology ( IF 3.6 ) Pub Date : 2020-02-09 , DOI: 10.1016/j.molimm.2020.01.025
Kaustav Majumder 1 , Yan Jin 1 , Hiroshi Shibata 2 , Yoshinori Mine 1
Affiliation  

Food protein-induced allergies are primarily aggravated due to imbalance immune responses. Earlier studies by different research groups have reported that the intervention of Lactobacillus pentosus (L. pentosus) S-PT84 can modulate T-helper (Th)1/Th2 balance through regulatory T cells and can effectively promote type 1 immunity by activating dendritic cells and natural killer cells, such biological activity makes L. pentosus S-PT84 a potential mediation in controlling food allergy. Thus, this study aimed to evaluate the effects of L. pentosus S-PT84 against egg ovalbumin (OVA)-induced allergic response in mice. BALB/c mice (n = 12/group) were sensitized with OVA (50 μg/mice) via intraperitoneal injection (IP) for four weeks and subsequently administered with three different doses of L. pentosus S-PT84 via pelleted diet. The allergenic status was assessed by clinical signs, serum histamine, mouse mast cell protease (MMCP) level, and antibody activity, cytokines level in splenocytes, and expression of T regulatory cells (T-regs) in blood. The intervention of L. pentosus S-PT84, precisely at the high dose (0.6 % L. pentosus S-PT84 in pelleted diet) group, significantly reduced the clinical allergenic symptoms and reduced the histamine and MMCP levels in serum. However, the intervention of L. pentosus S-PT84 did not affect the OVA-specific IgE, IgG concentration, but led to lower the total IgE and IgG titers, suggesting that the therapeutic effect of L. pentosus S-PT84 may be due to development of immune tolerance. Moreover, differences in the immune response were observed after L. pentosus S-PT84 intervention, as it significantly reduced the production of IL-4, IL-17, and increased the population of CD25+Foxp3+ cells. Thus, it can be concluded that the intervention of L. pentosus S-PT84 provides a potential therapeutic strategy to reduce the chicken egg OVA-induced allergic symptoms.

中文翻译:

戊糖乳杆菌S-PT84的口服干预减弱了对鸡蛋过敏的BALB / C小鼠模型的过敏反应。

由于免疫反应失衡,主要加剧了食物蛋白引起的过敏。多个研究小组的早期研究报告称,戊糖乳杆菌(S.PT84)的干预可以通过调节性T细胞调节T辅助(Th)1 / Th2平衡,并可以通过激活树突状细胞来有效促进1型免疫。天然杀伤细胞,这种生物活性使戊糖乳杆菌S-PT84成为控制食物过敏的潜在媒介。因此,本研究旨在评估戊糖乳杆菌S-PT84对卵卵白蛋白(OVA)诱导的小鼠过敏反应的影响。通过腹膜内注射(IP)对BALB / c小鼠(n = 12 /组)进行OVA致敏(50μg/小鼠)持续4周,然后通过成球状饮食给予三种不同剂量的戊糖乳杆菌S-PT84。通过临床体征,血清组胺,小鼠肥大细胞蛋白酶(MMCP)水平和抗体活性,脾细胞中的细胞因子水平以及血液中T调节细胞(T-regs)的表达来评估变应原状态。戊糖乳杆菌S-PT84的干预恰好在高剂量(颗粒状饮食中为0.6%戊糖乳杆菌S-PT84)组显着减轻了临床过敏性症状,并降低了血清中的组胺和MMCP水平。然而,戊糖乳杆菌S-PT84的干预并未影响OVA特异性IgE,IgG的浓度,但导致总的IgE和IgG滴度降低,这表明戊糖乳杆菌S-PT84的治疗作用可能是由于免疫耐受的发展。此外,戊糖乳杆菌S-PT84干预后观察到免疫反应的差异,因为它显着降低了IL-4的产生,IL-17,并增加了CD25 + Foxp3 +细胞的种群。因此,可以得出结论,戊糖乳杆菌S-PT84的干预提供了减轻鸡蛋OVA引起的过敏症状的潜在治疗策略。
更新日期:2020-02-20
down
wechat
bug