Realization of phototherapy on the big animal modal with orthotopic tumor is of considerable significance in view of its great clinical relevance to the human deep tumor treatment. Herein, near infrared (NIR)-active ZrN nanoparticles were chosen for both of photothermal and photodynamic purposes to achive the synergetic phototherapy on mice with subcutaneous tumor and even rabbits bearing with orthotopic tumor. Broad and strong photoabsorption, photosensitive ROS generation and photothermal effect of ZrN nanoparticles together made it to be ideal candidate for the effective tumor photoablation. Meanwhile, cell-cargo of macrophage enables targeted delivery of ZrN nanoparticles without influence on its photophysical properties. Resultantly, macrophage loaded ZrN could efficiently mediate synergetic phototherapeutic outcome as proved by complete removal of solid tumor from mice and rabbits. In this work, we also introduced B-mode ultrasonography and contrast-enhanced ultrasound technique for monitoring the evolution process of deep orthotopic tumor on rabbits post-treatment and confirmed the pathological changes of vascular degeneration and liquefaction necrosis post phototherapy.

中文翻译：

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细胞货物介导的ZrN纳米粒子对小鼠和兔子的协同光疗。

考虑到光疗法与人类深部肿瘤治疗的巨大临床联系，在具有原位肿瘤的大动物模态上实现光疗具有重要意义。在本文中，出于光热和光动力目的，选择了近红外（NIR）活性ZrN纳米粒子，以在具有皮下肿瘤的小鼠甚至患有原位肿瘤的兔子上实现协同光疗。ZrN纳米粒子具有广泛而强大的光吸收能力，光敏性ROS产生和光热效应，使其成为有效肿瘤光消融的理想候选者。同时，巨噬细胞的细胞货物能够靶向递送ZrN纳米颗粒而不会影响其光物理性质。结果，巨噬细胞中的ZrN可以有效地介导协同的光疗结果，这可以通过从小鼠和兔子体内完全清除实体瘤来证明。在这项工作中，我们还介绍了B型超声检查和对比增强超声技术，用于监测兔子治疗后原位深部原位肿瘤的演变过程，并确认光疗后血管变性和液化坏死的病理变化。