Active targeted drug delivery methods facilitate effective uptake of functionalized nanoparticles through receptor-mediated transcytosis. In recent years, albumin-nanoparticle interaction has been critically examined so that this functionalized nanoparticle can be efficiently loaded with drugs. The present investigation aims at understanding the adsorption of Bovine Serum Albumin (BSA) on Silver Nanoparticle (SNP) surface, preparation of soft conjugates (SC) and hard conjugates (HC) of BSA-functionalized SNP (SNP-BSA), and their interaction with curcumin (CUR). HC contains tightly bound BSA whereas SC involves tightly and loosely bound BSA. Increase in the hydrodynamic radii of conjugates was observed upon SNP incubation with increased concentration of BSA. Three different SNP-BSA conjugate ratios were selected to study their interaction with CUR. Fluorescence spectroscopy showed a strong association between CUR and SNP:BSA conjugates. However, binding varied with a change in the conjugate ratio. Circular Dichroism (CD)/Fourier Transform Infrared (FTIR) spectroscopy revealed the alterations in the secondary structure of BSA upon CUR binding to the conjugates. Zeta potential data indicated stable conjugate formation. CUR in SNP:BSA conjugate was found to have a higher half-life as compared to the control. We believe that this is the first biophysical characterization report of conjugates that can be effectively extrapolated for targeted drug delivery.

主动靶向药物递送方法有助于通过受体介导的胞吞作用有效摄取功能化的纳米颗粒。近年来，已经对白蛋白与纳米颗粒的相互作用进行了严格的检查，以使该功能化的纳米颗粒可以有效地负载药物。本研究旨在了解牛血清白蛋白（BSA）在银纳米颗粒（SNP）表面上的吸附，BSA功能化SNP（SNP-BSA）的软结合物（SC）和硬结合物（HC）的制备以及它们之间的相互作用与姜黄素（CUR）。HC包含紧密结合的BSA，而SC包含紧密和松散结合的BSA。用浓度增加的BSA孵育SNP后，观察到缀合物的流体动力学半径增加。选择三种不同的SNP-BSA共轭比率来研究它们与CUR的相互作用。荧光光谱显示CUR和SNP：BSA共轭物之间有很强的联系。但是，结合随结合率的变化而变化。圆二色谱（CD）/傅立叶变换红外（FTIR）光谱揭示了CUR与结合物结合后BSA二级结构的变化。Zeta电位数据表明稳定的缀合物形成。与对照相比，发现SNP：BSA共轭物中的CUR具有更高的半衰期。我们相信这是结合物的第一个生物物理表征报告，可以有效地推断出靶向药物的递送。圆二色谱（CD）/傅立叶变换红外（FTIR）光谱揭示了CUR与结合物结合后BSA二级结构的变化。Zeta电位数据表明稳定的缀合物形成。与对照相比，发现SNP：BSA共轭物中的CUR具有更高的半衰期。我们相信这是结合物的第一个生物物理表征报告，可以有效地推断出靶向药物的递送。圆二色谱（CD）/傅立叶变换红外（FTIR）光谱揭示了CUR与结合物结合后BSA二级结构的变化。Zeta电位数据表明稳定的缀合物形成。与对照相比，发现SNP：BSA共轭物中的CUR具有更高的半衰期。我们相信这是结合物的第一个生物物理表征报告，可以有效地推断出靶向药物的递送。