BACKGROUND Cucurbiturils (CB) are pumpkin-shaped macrocyclic molecules consisting of methylen-bridged glycoluril units. Because of their complexing characteristics, they can be used as drug containers for medical purposes. For future biomedical and dermal application of CB, the investigation of cell compatibility is essential. Little is known about the influence of CB on eukaryotic cells, especially on dermal keratinocytes. The structurally related cyclodextrins are known to induce cell death by apoptosis in HaCaT keratinocytes as well as hemolysis in erythrocytes. OBJECTIVE To examine cytotoxic effects of different CB. METHODS Different cytotoxicity tests were performed on HaCaT keratinocytes and erythrocytes incubated with CB[5], CB[6], and CB[7]. RESULTS CB[5] and CB[6] did not lead to cytotoxic reactions at high concentrations up to 30 mg/mL whereas incubation with CB[7] triggered apoptosis at a concentration of 3.75 mg/mL. None of the investigated CB caused hemolytic effects on erythrocytes. CONCLUSION These results confirm the high potential of CB as host-complexes for biomedical and dermal applications.

中文翻译：

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葫芦脲细胞和血液相容性的评价。

背景技术葫芦脲(CB)是由亚甲基桥联的甘脲单元组成的南瓜形大环分子。由于它们的络合特性，它们可以用作医疗用途的药物容器。对于 CB 的未来生物医学和皮肤应用，细胞相容性的研究是必不可少的。关于 CB 对真核细胞的影响，尤其是对真皮角质形成细胞的影响知之甚少。已知结构相关的环糊精通过 HaCaT 角质形成细胞中的细胞凋亡以及红细胞中的溶血诱导细胞死亡。目的考察不同CB的细胞毒作用。方法 对 HaCaT 角质形成细胞和与 CB[5]、CB[6] 和 CB[7] 孵育的红细胞进行不同的细胞毒性测试。结果 CB[5] 和 CB[6] 在高达 30 mg/mL 的高浓度下不会导致细胞毒性反应，而与 CB[7] 一起孵育在 3.75 mg/mL 的浓度下会引发细胞凋亡。所研究的CB均未对红细胞造成溶血作用。结论 这些结果证实了 CB 作为宿主复合物在生物医学和皮肤应用中的巨大潜力。