Background

Acute pancreatitis (AP) has a wide spectrum of severity and can be associated with considerable morbidity and mortality. Whether gut microbiota dysbiosis is associated with AP severity remains obscure.

Aims

We aim to investigate the differences in the alterations of gut microbiota in different grades of AP severity.

Methods

We collected clinical information and rectal swab samples from 80 individuals. The gut microbiota was tested by 16S rRNA gene sequencing, gut microbiota species composition analysis, difference analysis, random forest model prediction analysis, and gut microbiota species correlation network analysis.

Results

There was a different microbiota profile in different severity grades. Bacteroides, Escherichis-Shigella, and Enterococcus were dominant species in mild, moderately severe, and severe AP, respectively. Finegoldia was the most significantly increased and Blautia the most decreased species in mild AP. Anaerococcus was the most significantly increased and Eubacterium hallii the most decreased species in moderately severe AP. Enterococcus was the most significantly increased and Eubacterium hallii the most decreased species in severe AP. Finegoldia, Eubacterium_hallii, and Lachnospiraceae were potential diagnostic biomarkers for mild AP and Eubacterium_hallii and Anaerococcus for moderately severe AP. There was a positive interaction between Firmicutes and Bacteroidetes in mild AP.

Conclusions

The disturbed gut microbiota is different among grades of AP, suggesting their potential role in the progression of disease severity.

Graphic Abstract

There was a different microbiota profile in different severity grades. Bacteroides, Escherichis-Shigella, and Enterococcus were dominant gut microbiota species in MAP, MSAP, and SAP, respectively. Finegoldia was the most significantly increased and Blautia the most decreased gut microbiota species in MAP. Anaerococcus was the most significantly increased and Eubacterium hallii the most decreased species in MSAP. Enterococcus was the most significantly increased and Eubacterium hallii the most decreased species in SAP. Finegoldia, Eubacterium_hallii, and Lachnospiraceae were potential diagnostic biomarkers for MAP and Eubacterium_hallii and Anaerococcus for MSAP. There was a positive interaction between Firmicutes and Bacteroidetes in MAP.

中文翻译：

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通过严重程度不同的急性胰腺炎患者通过直肠拭子鉴定肠道功能障碍。

背景

急性胰腺炎（AP）的严重程度范围很广，可能与相当高的发病率和死亡率有关。肠道菌群失调是否与AP严重程度有关仍不清楚。

目的

我们的目的是调查不同级别的AP严重程度中肠道菌群变化的差异。

方法

我们收集了80位患者的临床信息和直肠拭子样本。通过16S rRNA基因测序，肠道菌群物种组成分析，差异分析，随机森林模型预测分析和肠道菌群物种相关网络分析对肠道菌群进行了测试。

结果

在不同的严重程度等级中，微生物群落的特征有所不同。拟杆菌，大肠杆菌-志贺氏菌和肠球菌分别是轻度，中度和重度AP中的优势种。Finegoldia是最显著上升，Blautia轻度AP最下降的品种。Anaerococcus是最显著上升，真杆菌霍氏真杆菌最下降种中度AP。在严重AP中，肠球菌增加最多，而Halii真细菌减少最多。Finegoldia，优生细菌和鞭毛藻科是轻度AP的潜在诊断生物标志物，优生细菌和厌氧球菌是中度重度AP的潜在诊断标志物。在轻度AP中，纤毛虫和拟杆菌之间存在正相互作用。

结论

在不同等级的AP中，肠道微生物区系不同，表明它们在疾病严重程度发展中的潜在作用。

图形摘要

在不同的严重程度等级中，微生物群落的特征有所不同。拟杆菌，大肠杆菌-志贺氏菌和肠球菌分别是MAP，MSAP和SAP中主要的肠道菌群。Finegoldia是最显著上升，Blautia最下降的MAP肠道菌群种类。Anaerococcus是最显著上升，真杆菌霍氏真杆菌最下降种MSAP。在SAP中，肠球菌的增幅最大，而哈氏真细菌的降幅最大。Finegoldia，真细菌和钩毛藻科是MAP的潜在诊断生物标志物，真细菌和厌氧球菌对于MSAP是潜在的诊断生物标志物。MAP中的Firmicutes和Bacteroidetes之间存在积极的相互作用。