Lateral flow assays (LFA) are an affordable, easy-to-use, qualitative rapid test for clinical diagnosis in nonlaboratory environments and low-resource facilities. The control line of these tests is very important to provide a valid result, confirming that the platform operates correctly. A clear, nondiffused line is desirable. The number of colored nanoparticles that reach the control line in a positive test can be very small, and they should all be trapped efficiently by the molecules adsorbed there. In this work, we proposed the use of robust biotinylated dendrimers of two different generations as signal amplifiers in control lines of LFA, able to react with streptavidin-modified gold nanoparticles. Besides the synthesis and characterization, the analytical performance as control lines will be studied, and their response will be compared with other commercially available biotinylated molecules. Finally, the utility of the dendrimer implemented in a NALF (Nucleic Acid Lateral Flow) strip was also demonstrated for detection of the amplicons obtained by double-tagging PCR (polymerase chain reaction) for the detection of E. coli as a model of foodborne pathogen.

中文翻译：

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在核酸侧向流动测试中，生物素化的磷树枝状大分子作为对照。

横向流动分析（LFA）是一种经济实惠，易于使用的定性快速测试，可用于在非实验室环境和资源贫乏的设施中进行临床诊断。这些测试的控制线对于提供有效结果，确认平台正确运行非常重要。希望有一条清晰，无扩散的线条。在阳性试验中，到达控制线的有色纳米粒子的数量可能非常少，它们都应该被吸附在其中的分子有效地捕获。在这项工作中，我们建议使用两个不同世代的坚固的生物素化树状大分子作为LFA对照系中的信号放大器，能够与链霉亲和素修饰的金纳米颗粒反应。除了合成和表征外，还将研究作为控制线的分析性能，并将其反应与其他市售生物素化分子进行比较。最后，还证明了在NALF（核酸横向流动）条中实施的树状聚合物可用于检测通过双标签PCR（聚合酶链反应）获得的扩增子，用于检测食源性病原体模型的大肠杆菌。