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miRNA-1-3p is an early embryonic male sex-determining factor in the Oriental fruit fly Bactrocera dorsalis.
Nature Communications ( IF 16.6 ) Pub Date : 2020-02-18 , DOI: 10.1038/s41467-020-14622-4
Wei Peng 1, 2 , Shuning Yu 1, 2 , Alfred M Handler 3 , Zhijian Tu 4 , Giuseppe Saccone 5 , Zhiyong Xi 6 , Hongyu Zhang 1, 2
Affiliation  

Regulation of male sexual differentiation by a Y chromosome-linked male determining factor (M-factor) is one of a diverse array of sex determination mechanisms found in insects. By deep sequencing of small RNAs from Bactrocera dorsalis early embryos, we identified an autosomal-derived microRNA, miR-1-3p, that has predicted target sites in the transformer gene (Bdtra) required for female sex determination. We further demonstrate by both in vitro and in vivo tests that miR-1-3p suppresses Bdtra expression. Injection of a miR-1-3p mimic in early embryos results in 87-92% phenotypic males, whereas knockdown of miR-1-3p by an inhibitor results in 67-77% phenotypic females. Finally, CRISPR/Cas9-mediated knockout of miR-1-3p results in the expression of female-specific splice variants of Bdtra and doublesex (Bddsx), and induced sex reversal of XY individuals into phenotypic females. These results indicate that miR-1-3p is required for male sex determination in early embryogenesis in B. dorsalis as an intermediate male determiner.

中文翻译:

miRNA-1-3p是东方果蝇小实蝇Bactrocera dorsalis中的早期胚胎男性性别决定因素。

Y染色体连锁的雄性决定因子(M因子)对雄性分化的调控是在昆虫中发现的多种性别决定机制之一。通过对背实双歧杆菌早期胚中的小RNA进行深度测序,我们鉴定出了常染色体来源的microRNA miR-1-3p,它预测了确定雌性所需的转化基因(Bdtra)中的目标位点。我们通过体外和体内试验进一步证明了miR-1-3p抑制Bdtra表达。在早期胚胎中注射miR-1-3p模拟物会导致87-92%的表型雄性,而通过抑制剂敲低miR-1-3p会导致67-77%的表型雌性。最后,CRISPR / Cas9介导的miR-1-3p敲除导致女性特异性Bdtra和doublesex(Bddsx)剪接变体的表达,并导致XY个体的性别逆转为表型女性。这些结果表明,miR-1-3p是在背背双歧杆菌的早期胚胎发生中确定男性性别的必需条件,它是中间的男性确定因子。
更新日期:2020-02-18
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