On the basis of solvothermal reaction, a new porous Cu(II) metal-organic framework (MOF), {[(Cu(L)₂(H₂O)₂](DMF)₄)_n (namely 1), can be formed via self-assembly of 3-(1H-pyrazol-4-yl)pyridine (namely HL) and Cu(II) ion in water and DMF mixed solvent. We characterized the MOF 1 via elemental analysis, powder X-ray diffraction (PXRD) and single crystal X-ray diffraction, thermogravimetric analysis (TGA) as well as Fourier-transform infrared spectra (FT-IR). In addition, MOF 1 can be used as a solvent free multiphase catalyst for cyanidation due to its open Cu(II) metal sites as well as a larger number of N atoms can be utilized as Lewis basic sites. In connection to these, the catalysis mechanism has also been discussed in detail. To explore the inhibitory function of compound 1a on Hey ovarian cancer cells viability, the CCK-8 was carried out, and the results revealed that compound 1a had good anti-cancer activity in vitro with the IC₅₀ value of 2.81 ± 0.17 μg/mL. Besides, the anti-tumor mechanism of compound 1a was revealed by apoptosis and ROS detection, the results suggested that compound 1a anti-tumor activity owing to the inducing of cell apoptosis by increasing the ROS accumulation.

在溶剂热反应的基础上，可以将新的多孔Cu（II）金属有机骨架（MOF）{[（Cu（L）₂（H ₂ O）₂ ]（DMF）₄）_n（即1）为通过3-（1H-吡唑-4-基）吡啶（即HL）和Cu（II）离子在水和DMF混合溶剂中的自组装形成。我们通过元素分析，粉末X射线衍射（PXRD）和单晶X射线衍射，热重分析（TGA）以及傅立叶变换红外光谱（FT-IR）对MOF 1进行了表征。此外，MOF 1由于其开放的Cu（II）金属位点以及大量的N原子可用作Lewis碱性位点，因此可以用作无溶剂的氰化多相催化剂。结合这些，还详细讨论了催化机理。为了研究化合物1a对Hey卵巢癌细胞生存能力的抑制作用，进行了CCK-8的研究，结果表明化合物1a具有良好的体外抗癌活性，IC ₅₀值为2.81±0.17μg/ mL。 。此外，通过凋亡和ROS检测揭示了化合物1a的抗肿瘤机制，结果表明化合物1a具有抗肿瘤作用。 由于通过增加ROS的积累诱导细胞凋亡而产生抗肿瘤活性。