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A safety type of genetically engineered bacterium that degrades chemical pesticides.
AMB Express ( IF 3.7 ) Pub Date : 2020-02-18 , DOI: 10.1186/s13568-020-00967-y
Qin Li 1, 2 , Jing Li 1 , Ke-Lai Kang 1 , Yi-Jun Wu 1
Affiliation  

Chemical pesticides are used widely and their residues are found in the environment. Pesticide pollution has become a global problem. To find an economical, effective and safety way to degrade residues of pesticides in environment, we constructed a genetically engineered bacterium (GEB) having the ability to degrade pesticides, emit green fluorescence and has a containment system by using a dual plasmid expression system. One plasmid contains the genes of enhanced green fluorescent protein (EGFP) and carboxylesterase B1 (CarE B1), which were cloned downstream of lambda PL promoter and expressed constitutively. The gene of CarE B1 encodes an insect-detoxifying enzyme possessing the degradability to organochloride pesticides, organophosphorus pesticides, carbamates, and pyrethoid insecticides. The other is the conditional suicide plasmid for containment system, in which the lethal gene used was the nuclease gene of Serratia marcescens without the leader-coding sequence and was placed downstream of T7 promoter. The GEB has wide prospects of application on cleanup of pesticide residues with its degradability to several pesticides and containment system.

中文翻译:

一种安全类型的可降解化学农药的基因工程细菌。

化学农药被广泛使用,其残留物在环境中被发现。农药污染已成为全球性问题。为了找到一种在环境中降解农药残留的经济,有效和安全的方法,我们构建了具有降解农药,发出绿色荧光的基因工程细菌(GEB),并使用双重质粒表达系统构建了一个遏制系统。一种质粒包含增强的绿色荧光蛋白(EGFP)和羧酸酯酶B1(CarE B1)的基因,它们被克隆到λPL启动子的下游并组成性表达。CarE B1基因编码一种昆虫解毒酶,具有可降解为有机氯化物农药,有机磷农药,氨基甲酸酯和拟除虫菊酯类农药的能力。另一个是用于封闭系统的条件自杀质粒,其中使用的致死基因是无前导序列的粘质沙雷氏菌的核酸酶基因,并置于T7启动子的下游。GEB具有可降解为多种农药和遏制系统的广阔前景,可用于清除农药残留。
更新日期:2020-02-18
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