We introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling the cellular localization of GFP fusion proteins, the engineered nanobody allows interrogation of their roles in basic biological processes, an approach that should be applicable to numerous previously described GFP fusions. We also outline how the binding affinities of other nanobodies can be controlled by small molecules.

中文翻译：

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纳米抗体的化学遗传学控制

我们介绍了一种工程纳米体，其对绿色荧光蛋白 (GFP) 的亲和力可以通过小分子打开和关闭。通过控制 GFP 融合蛋白的细胞定位，工程纳米体允许询问它们在基本生物过程中的作用，这种方法应该适用于许多先前描述的 GFP 融合。我们还概述了如何通过小分子控制其他纳米抗体的结合亲和力。