Despite efforts to visualize the spatio-temporal dynamics of single messenger RNAs, the ability to precisely control their function has lagged. This study presents an optogenetic approach for manipulating the localization and translation of specific mRNAs by trapping them in clusters. This clustering greatly amplified reporter signals, enabling endogenous RNA-protein interactions to be clearly visualized in single cells. Functionally, this sequestration reduced the ability of mRNAs to access ribosomes, markedly attenuating protein synthesis. A spatio-temporally resolved analysis indicated that sequestration of endogenous β-actin mRNA attenuated cell motility through the regulation of focal-adhesion dynamics. These results suggest a mechanism highlighting the indispensable role of newly synthesized β-actin protein for efficient cell migration. This platform may be broadly applicable for use in investigating the spatio-temporal activities of specific mRNAs in various biological processes.

中文翻译：

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活细胞中mRNA定位和翻译的光遗传学控制。

尽管努力可视化单个信使RNA的时空动态，但精确控制其功能的能力却落后了。这项研究提出了一种光遗传学方法，通过将它们捕获在簇中来操纵它们的定位和翻译。这种聚集极大地放大了报告信号，使内源性RNA-蛋白质相互作用在单个细胞中清晰可见。从功能上讲，这种螯合作用降低了mRNA进入核糖体的能力，从而显着减弱了蛋白质的合成。时空分辨的分析表明，内源性β-肌动蛋白mRNA的螯合通过调节粘着动力学来减弱细胞运动。这些结果表明了一种机制，突出了新合成的β-肌动蛋白在有效细胞迁移中不可或缺的作用。