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Dynamic screening and printing of single cells using a microfluidic chip with dual microvalves.
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-02-17 , DOI: 10.1039/d0lc00040j
Chang Chen 1 , Dong Xu , Siwei Bai , Zhihang Yu , Yonggang Zhu , Xiao Xing , Huaying Chen
Affiliation  

Inoculation of single cells into separate culture chambers is one of the key requirements in single-cell analysis. This paper reports an innovative microfluidic chip integrating two pneumatic microvalves to screen and print single cells onto a well plate. The upper and lower size limits of cells can be dynamically controlled by regulating the deformation of two adjacent microvalves. Numerical simulations were employed to systematically study the influence of membrane dimensions and pressure on the deflection of a valve. A mathematical model was then modified to predict the size of cells captured by a microvalve at various pressures. The membrane deflection was further studied using confocal imaging. The critical pressure trapping beads of various sizes was experimentally determined. These experiments validated the accuracy of both numerical simulations and the mathematical model. Furthermore, single beads and endothelial cells with the desired size range were screened using dual valves and printed onto well plates with 100% efficiency. Viability studies suggested that the screening process had no significant impact on cells. This device enables dynamic regulation of both the lower and the upper size limits of cells for printing. It has significant application potential in inoculating cells with desired sizes for various fields such as clonal expansion, monoclonality development and single-cell genomic studies.

中文翻译:

使用带有双微阀的微流控芯片动态筛选和打印单细胞。

将单细胞接种到单独的培养室中是单细胞分析的关键要求之一。本文报道了一种创新的微流控芯片,该芯片集成了两个气动微阀,以将单个细胞筛选并打印到孔板上。可以通过调节两个相邻微型阀的变形来动态控制单元的大小上限和下限。数值模拟被用来系统地研究膜的尺寸和压力对阀门挠度的影响。然后修改数学模型,以预测在不同压力下微阀捕获的细胞大小。使用共聚焦成像进一步研究膜的偏转。实验确定了各种尺寸的临界压力捕获珠。这些实验验证了数值模拟和数学模型的准确性。此外,使用双阀筛选具有所需大小范围的单珠和内皮细胞,并以100%的效率印刷到孔板上。活力研究表明,筛选过程对细胞没有显着影响。该设备可以动态调节打印单元格的大小上限和下限。它在接种具有所需大小的细胞以用于各个领域(如克隆扩增,单克隆抗体开发和单细胞基因组研究)方面具有巨大的应用潜力。活力研究表明,筛选过程对细胞没有显着影响。该设备可以动态调节打印单元格的大小上限和下限。它在接种具有所需大小的细胞以用于各个领域(如克隆扩增,单克隆抗体开发和单细胞基因组研究)方面具有巨大的应用潜力。活力研究表明,筛选过程对细胞没有显着影响。该设备可以动态调节打印单元格的大小上限和下限。它在接种具有所需大小的细胞以用于各个领域(如克隆扩增,单克隆抗体开发和单细胞基因组研究)方面具有巨大的应用潜力。
更新日期:2020-02-17
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