Powdery mildew is a serious fungal disease of wheat caused by Blumeria graminis f. sp. tritici. Chromosome 5U of Aegilops triuncialis carrying powdery mildew resistance was induced to recombine with chromosome 5A of wheat using ph1b deletion line. The resulting recombinant progeny lines were tested for resistance and screened with molecular markers. Among 367 progeny lines 8 were immune to powdery mildew, 53 were moderately resistant, 231 moderately susceptible and 75 highly susceptible. Screening of 5U/5A recombinant lines with 5A specific SSR markers indicated that a major powdery mildew resistance gene from A. triuncialis was introgressed on the long arm of wheat chromosome 5A, along with two other loci, one located in the terminal end of the same arm and the other on the short arm of 5A. Meiotic metaphase-I pairing indicated that chromosome pairing in the resistant recombinant lines was regular with 21 bivalents and high seed set. The DNA markers associated with the introgressions can be used in marker assisted selection for improved powdery mildew resistance in wheat.

中文翻译：

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通过诱导的同源配对将黑麦草（Aegilops triuncialis）的白粉病抗性渗入小麦

白粉病是由Blumeria graminis f引起的一种严重的小麦真菌病。sp。小麦。的染色体5U三芒山羊草携带抗白粉病诱导用小麦染色体5A重组PH1B删除线。测试所得的重组子代系的抗性并用分子标记进行筛选。在367个后代系中，有8个对白粉病免疫，53个具有中等抗性，231个中等敏感，75个高度敏感。用5A特异SSR标记筛选5U / 5A重组品系表明，来自曲霉的主要白粉病抗性基因该基因与小麦5A染色体长臂以及其他两个基因座一起渗入，一个位于同一臂的末端，另一个位于5A的短臂上。减数分裂-I配对表明抗性重组品系中的染色体配对是规则的，具有21个二价和高结实率。与基因渗入相关的DNA标记可用于标记辅助选择中，以改善小麦的白粉病抗性。