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Population genetics and taxonomic signatures of wild Tilapia in Japan based on mitochondrial DNA control region analysis
Hydrobiologia ( IF 2.6 ) Pub Date : 2020-02-17 , DOI: 10.1007/s10750-020-04203-3
Patrick Senam Kofi Fatsi , Shaharior Hashem , Atsuya Kodama , Ebenezer Koranteng Appiah , Hidetoshi Saito , Koichiro Kawai

Non-native tilapia species were repeatedly introduced to Asian and South American countries because of their adaptiveness to new environments and new environmental conditions. Such founder populations rapidly adapted by evolving outstanding ecological and physiological features, making them highly successful invaders throughout the tropical and subtropical regions. In Japan, nine tilapia species were introduced. In recent years, several established populations were introgressed by accidental release of aquaculture strains of Oreochromis niloticus, causing reductions in population sizes, genetic diversity, and genetic perturbation upon introgressive hybridization. This study aimed to assess the levels of genetic integrity, genetic population structure, and biogeographic relationships of wild tilapia species found in Japan. Phylogenetic and phylogeographic analysis based on mtDNA control region sequences yielded 52 distinct haplotypes, indicating significant differences in genetic variability among the analyzed populations. Analysis of molecular variance showed high levels of variation within populations, except for tilapia species representing reproductively incompatible taxonomic units to O. niloticus aquaculture stains. Overall, a significant genetic structure emerged among the 11 populations analyzed. Our results indicated that accidental release of O. niloticus via aquaculture contributed significantly to the current genetic differentiation among populations within a few generations, showing clear signals of mtDNA introgression across species boundaries.

中文翻译:

基于线粒体DNA控制区分析的日本野生罗非鱼种群遗传学和分类特征

非本地罗非鱼物种因其对新环境和新环境条件的适应性而被反复引入亚洲和南美国家。这些始祖种群通过进化出突出的生态和生理特征迅速适应,使它们成为整个热带和亚热带地区非常成功的入侵者。在日本,引进了九种罗非鱼。近年来,由于尼罗罗非鱼水产养殖菌株的意外释放,一些已建立的种群被渗入,导致种群规模、遗传多样性和渗入杂交后的遗传扰动减少。本研究旨在评估在日本发现的野生罗非鱼物种的遗传完整性水平、遗传种群结构和生物地理关系。基于 mtDNA 控制区序列的系统发育和系统地理学分析产生了 52 种不同的单倍型,表明所分析种群之间的遗传变异性存在显着差异。分子方差分析显示种群内的变异水平很高,但代表与尼罗罗非鱼水产养殖污渍在生殖上不相容的分类单位的罗非鱼物种除外。总体而言,在分析的 11 个种群中出现了显着的遗传结构。我们的结果表明,通过水产养殖意外释放 O. niloticus 对几代内种群之间的当前遗传分化做出了重大贡献,显示出跨物种边界的 mtDNA 基因渗入的明确信号。表明所分析的种群之间的遗传变异性存在显着差异。分子方差分析显示种群内的变异水平很高,但代表与尼罗罗非鱼水产养殖污渍在生殖上不相容的分类单位的罗非鱼物种除外。总体而言,在分析的 11 个种群中出现了显着的遗传结构。我们的结果表明,通过水产养殖意外释放 O. niloticus 对几代内种群之间的当前遗传分化做出了重大贡献,显示出跨物种边界的 mtDNA 基因渗入的明确信号。表明所分析的种群之间的遗传变异性存在显着差异。分子方差分析显示种群内的变异水平很高,但代表与尼罗罗非鱼水产养殖污渍在生殖上不相容的分类单位的罗非鱼物种除外。总体而言,在分析的 11 个种群中出现了显着的遗传结构。我们的结果表明,通过水产养殖意外释放 O. niloticus 对几代内种群之间的当前遗传分化做出了重大贡献,显示出跨物种边界的 mtDNA 基因渗入的明确信号。除了代表与尼罗罗非鱼 (O. niloticus) 水产养殖污渍在生殖上不相容的分类单位的罗非鱼物种。总体而言,在分析的 11 个种群中出现了显着的遗传结构。我们的结果表明,通过水产养殖意外释放 O. niloticus 对几代内种群之间的当前遗传分化做出了重大贡献,显示出跨物种边界的 mtDNA 基因渗入的明确信号。除了代表与尼罗罗非鱼 (O. niloticus) 水产养殖污渍在生殖上不相容的分类单位的罗非鱼物种。总体而言,在分析的 11 个种群中出现了显着的遗传结构。我们的结果表明,通过水产养殖意外释放 O. niloticus 对几代内种群之间的当前遗传分化做出了重大贡献,显示出跨物种边界的 mtDNA 基因渗入的明确信号。
更新日期:2020-02-17
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