Recently, researches have confirmed the crucial role of inflammatory response in Ca‐P ceramic‐induced osteogenesis, however, the underlying mechanism has not yet been fully understood. In this study, BCP and β‐TCP ceramics were used as material models to investigate the effect of physicochemical properties on inflammatory response in vitro. The results showed that BCP and β‐TCP could support macrophages attachment, proliferation, and spreading favorably, as well as promote gene expressions of inflammatory related cytokines (IL‐1, IL‐6, MCP‐1, and TNF‐α) and growth factors (TGF‐β, FGF, PDGF, VEGF, IGF, and EGF). BCP showed a facilitating function on the gene expressions earlier than β‐TCP. Further coculture experiments performed in vitro demonstrated that the CMs containing various increased cytokines for macrophages pre‐culture could significantly promote MSCs osteogenic differentiation, which was confirmed by the gene expressions of osteogenic specific markers and the intracellular OCN product accumulation under the stimulation of BCP and β‐TCP ceramics. Further evidence was found from the formation of mineralized nodules in BCM and TCM. In addition, this study showed a concise relationship between Ca‐P ceramic induced inflammation and its osteoinductivity that the increased cytokines and growth factors from macrophages could promote MSCs osteogenic differentiation.

中文翻译：

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磷酸钙陶瓷在间充质干细胞成骨分化中调节炎症的积极作用。

最近，研究证实了炎症反应在钙磷陶瓷诱导的成骨中的关键作用，但其潜在机制尚未完全清楚。本研究以 BCP 和 β-TCP 陶瓷为材料模型，研究理化性质对体外炎症反应的影响。结果表明，BCP和β-TCP可以促进巨噬细胞的附着、增殖和扩散，促进炎症相关细胞因子（IL-1、IL-6、MCP-1和TNF-α）的基因表达和生长因子（TGF-β、FGF、PDGF、VEGF、IGF 和 EGF）。BCP 对基因表达的促进作用早于 β-TCP。体外进行的进一步共培养实验表明，含有用于巨噬细胞预培养的各种增加细胞因子的CM可以显着促进MSCs的成骨分化，这通过成骨特异性标志物的基因表达和BCP和β刺激下细胞内OCN产物的积累得到证实。 -TCP陶瓷。从 BCM 和 TCM 中矿化结核的形成中发现了进一步的证据。此外，该研究表明，Ca-P 陶瓷诱导的炎症与其骨诱导性之间存在简洁的关系，即巨噬细胞中增加的细胞因子和生长因子可以促进 MSCs 的成骨分化。BCP和β-TCP陶瓷刺激下成骨特异性标志物的基因表达和细胞内OCN产物的积累证实了这一点。从 BCM 和 TCM 中矿化结核的形成中发现了进一步的证据。此外，该研究表明，Ca-P 陶瓷诱导的炎症与其骨诱导性之间存在简明的关系，即巨噬细胞中增加的细胞因子和生长因子可以促进 MSCs 的成骨分化。BCP和β-TCP陶瓷刺激下成骨特异性标志物的基因表达和细胞内OCN产物的积累证实了这一点。从 BCM 和 TCM 中矿化结核的形成中发现了进一步的证据。此外，该研究表明，Ca-P 陶瓷诱导的炎症与其骨诱导性之间存在简明的关系，即巨噬细胞中增加的细胞因子和生长因子可以促进 MSCs 的成骨分化。