Optimal combination of stem cells and biocompatible support material is a promising strategy for successful tissue engineering. The required differentiation of stem cells is crucial for functionality of engineered tissues and can be regulated by chemical and physical cues. Here we examined how boundary cap neural crest stem cells (bNCSCs) are affected when cultured in the same medium, but on collagen‐ or laminin‐polyacrylamide (PAA) scaffolds of different stiffness (0.5, 1, or ~7 kPa). bNCSCs displayed marked differences in their ability to attach, maintain a large cell population and differentiate, depending on scaffold stiffness. These findings show that the design of physical cues is an important parameter to achieve optimal stem cell properties for tissue repair and engineering.

中文翻译：

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支架特性对体外边界帽神经嵴干细胞粘附和维持的影响。

干细胞和生物相容性支持材料的最佳组合是成功组织工程的有前途的策略。干细胞所需的分化对于工程组织的功能至关重要，并且可以通过化学和物理线索进行调节。在这里，我们研究了边界帽神经嵴干细胞 (bNCSCs) 在相同培养基中培养时如何受到影响，但在不同刚度 (0.5、1 或 ~7 kPa) 的胶原蛋白或层粘连蛋白聚丙烯酰胺 (PAA) 支架上。bNCSCs 在附着能力、维持大量细胞群和分化能力方面表现出显着差异，这取决于支架刚度。这些发现表明，物理线索的设计是实现组织修复和工程优化干细胞特性的重要参数。