Controlling the conformation and function of biomolecules via photo-regulators holds great promise as a spatiotemporally controllable tool for disease control. In addition, introducing photo-regulators into biomolecules has also found applications in constructing smart nanomaterials. In spite of the astonishing advances that have taken place in the past few years, realizing highly controllable and efficient regulation over the conformation and function of biomolecules under physiological conditions is still challenging. Herein, sulfonated pyrene, SPy, was synthesized and used as a photo-regulator to control the looping of single-stranded DNAs (ssDNAs) in aqueous solution. Due to its water solubility, SPy merits use in the study of biomolecules in aqueous solution. The looping of the doubly SPy-modified ssDNAs is stimulated by irradiation and regulated by SPy. Photo-ionization generates the radical cation of SPy (SPy•+ ). The association of SPy•+ with its neutral counterpart of SPy gives rise to the dimer radical cation of SPy (SPy2•+ ). During the association process, the stabilization energy released to form SPy2•+ provides a driving force for the looping of ssDNAs. Conversely, the formed loop conformations were trapped by the formation of SPy2•+, allowing the looping dynamics to be investigated. The results shown herein suggest the potential of SPy as a photo-regulator for regulating the conformation and function of biomolecules under physiological condition.

中文翻译：

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磺化P作为单链DNA环的光调节剂。

通过光调节剂控制生物分子的构象和功能具有广阔的前景，可作为时空可控的疾病控制工具。另外，将光调节剂引入生物分子中也发现了在构建智能纳米材料中的应用。尽管在过去几年中取得了令人惊讶的进步，但是在生理条件下实现对生物分子的构象和功能的高度可控和有效的调节仍然是挑战。本文合成了磺化pyr SPy，并用作光调节剂来控制水溶液中单链DNA（ssDNAs）的环化。由于其水溶性，SPy值得用于研究水溶液中的生物分子。SPy修饰双倍SPy修饰的ssDNA的环。光电离产生SPy的自由基阳离子（SPy•+）。SPy•+与中性的SPy缔合产生SPy（SPy2•+）的二聚自由基阳离子。在缔合过程中，释放形成SPy2•+的稳定能为ssDNA的环化提供了驱动力。相反，形成的环构象被SPy2•+的形成所俘获，从而可以研究环动力学。本文显示的结果表明SPy作为光调节剂在生理条件下调节生物分子的构象和功能的潜力。SPy•+与中性的SPy缔合产生SPy（SPy2•+）的二聚自由基阳离子。在缔合过程中，释放形成SPy2•+的稳定能为ssDNA的环化提供了驱动力。相反，形成的环构象被SPy2•+的形成所俘获，从而可以研究环动力学。本文显示的结果表明SPy作为光调节剂在生理条件下调节生物分子的构象和功能的潜力。SPy•+与中性的SPy缔合产生SPy（SPy2•+）的二聚自由基阳离子。在缔合过程中，释放形成SPy2•+的稳定能为ssDNA的环化提供了驱动力。相反，形成的环构象被SPy2•+的形成所俘获，从而可以研究环动力学。本文显示的结果表明SPy作为光调节剂在生理条件下调节生物分子的构象和功能的潜力。